A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens.

A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Research Abstract Details 

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A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Abstract Text:

Bharesh K Chauhan,Nathan A Reed,Ying Yang, ,Lixing Reneker,Melinda K Duncan,Ales Cvekl,

BACKGROUND: Pax6 is a transcription factor that is required for induction, growth, and maintenance of the lens; however, few direct target genes of Pax6 are known. RESULTS: In this report, we describe the results of a cDNA microarray analysis of lens transcripts from transgenic mice over-expressing Pax6 in lens fibre cells in order to narrow the field of potential direct Pax6 target genes. This study revealed that the transcript levels were significantly altered for 508 of the 9700 genes analysed, including five genes encoding the cell adhesion molecules beta1-integrin, JAM1, L1 CAM, NCAM-140 and neogenin. Notably, comparisons between the genes differentially expressed in Pax6 heterozygous and Pax6 over-expressing lenses identified 13 common genes, including paralemmin, GDIbeta, ATF1, Hrp12 and Brg1. Immunohistochemistry and Western blotting demonstrated that Brg1 is expressed in the embryonic and neonatal (2-week-old) but not in 14-week adult lenses, and confirmed altered expression in transgenic lenses over-expressing Pax6. Furthermore, EMSA demonstrated that the BRG1 promoter contains Pax6 binding sites, further supporting the proposition that it is directly regulated by Pax6. CONCLUSIONS: These results provide a list of genes with possible roles in lens biology and cataracts that are directly or indirectly regulated by Pax6.

A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Publishing Authors By Initials

BK Chauhan,NA Reed,Y Yang,L ,L Reneker,MK Duncan,A Cvekl,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Genes to cells : devoted to molecular & cellular m

VOLUME: 7

Page Numbers: 1267-83

Journal Abbreviation: Genes Cells

ISSN: 1356-9597

DAY: 26

MONTH: Dec

YEAR: 2002

A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Information

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LANGUAGE: eng

NlmUniqueID: 9607379

A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Information

Substance Name: Smarca4 protein, mouse

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens.

AFFILIATION: The Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Country: England

AGENCY: United States NEI

GRANT: R01 EY012221-04

ACRONYM: EY

MEDLINETA: Genes Cells

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