A Col9a1 enhancer element activated by two interdependent SOX9 dimers.

A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Research Abstract Details 

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A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Abstract Text:

Mary Ann Genzer,Laura C Bridgewater,

The transcription factor SOX9 plays a critical role in chondrogenesis as well as in sex determination. Previous work has suggested that SOX9 functions as a DNA-dependent dimer when it activates genes involved in chondrogenesis, but functions as a monomer to activate genes involved in sex determination. We present evidence herein for a third binding configuration through which SOX9 can activate transcription. We have identified four separate SOX consensus sequences in a COL9A1 collagen gene enhancer. The sites are arranged as two pairs, and each pair is similar to previously discovered dimeric SOX9 binding sites. Increasing the spacing between the pairs of sites eliminated enhancer activity in chondrocytic cells, as did the mutation of any one of the four sites. The COL9A1 enhancer is ordinarily inactive in 10T1/2 cells, but cotransfection with a SOX9 expression plasmid was sufficient to activate the enhancer, and mutation of any one of the four sites reduced responsiveness to SOX9 overexpression. These results suggest a novel mechanism for transcriptional activation by SOX9, in which two SOX9 dimers that are bound at the two pairs of sites are required to interact with one another, either directly or indirectly, in order to produce a functional transcriptional activation complex.

A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Publishing Authors By Initials

MA Genzer,LC Bridgewater,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 1178-86

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 30

MONTH: 01

YEAR: 2007

A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Information

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LANGUAGE: eng

NlmUniqueID: 411011

A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: A Col9a1 enhancer element activated by two interdependent SOX9 dimers. Information

Substance Name: sox-9 transcription factor

Registry Number: 0

Grant and Affiliation Information for A Col9a1 enhancer element activated by two interdependent SOX9 dimers.

AFFILIATION: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah 84602, USA.

Country: England

AGENCY: United States NIAMS

GRANT: AR48839

ACRONYM: AR

MEDLINETA: Nucleic Acids Res

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