A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation.

A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Research Abstract Details 

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A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Abstract Text:

Barton Wicksteed,Yuji Uchizono,Cristina Alarcon,Jill F McCuaig,Anath Shalev,Christopher J Rhodes,

Insulin production in pancreatic beta cells is predominantly regulated through glucose control of proinsulin translation. Previously, this was shown to require sequences within the untranslated regions (UTRs) of the preproinsulin (ppI) mRNA. Here, those sequences were found to be sufficient for specific glucose-regulated proinsulin translation. Furthermore, an element 40-48 bp from the 5' end of the ppI mRNA specifically bound a factor present in islets of Langerhans. Glucose-responsive factor binding to this cis-element exhibited temporal and glucose-concentration-dependent patterns that paralleled proinsulin biosynthesis. Mutating this cis-element abolished the ability of ppI mRNA UTRs to confer glucose regulation upon translation. Like the rat 5'UTR, the human ppI 5'UTR conferred glucose regulation of translation. However alternative splicing of the human 5'UTR that disrupts the cis-element abolished glucose-regulated translation. These data indicate that glucose regulation of cis-element/trans-acting factor interaction is a key component of the mechanism by which glucose regulates insulin production.

A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Publishing Authors By Initials

B Wicksteed,Y Uchizono,C Alarcon,JF McCuaig,A Shalev,CJ Rhodes,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cell metabolism

VOLUME: 5

Page Numbers: 221-7

Journal Abbreviation: Cell Metab.

ISSN: 1550-4131

DAY: 3

MONTH: Mar

YEAR: 2007

A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101233170

A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation. Information

Substance Name: Proinsulin

Registry Number: 9035-68-1

Grant and Affiliation Information for A cis-element in the 5' untranslated region of the preproinsulin mRNA (ppIGE) is required for glucose regulation of proinsulin translation.

AFFILIATION: Comprehensive Diabetes Center, Section of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Chicago, IL 60637, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK56010

ACRONYM: DK

MEDLINETA: Cell Metab

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