A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S).

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Abstract Text:

K W Clara Sze,Harshal H Kshirsagar,Mahesh Venkatachalam,Shridhar K Sathe,K W Clara Sze,Harshal H Kshirsagar,Mahesh Venkatachalam,Shridhar K Sathe,

Soybean glycinin (11S) and beta-conglycinin (7S) were subjected to select chemical treatments at various concentrations and resulting changes in protein structures were investigated by circular dichroism (CD) and fluorescence spectrometry. Fluorescence quenching results indicated that urea >/=3 M caused significant unfolding of 11S, but not that of 7S. GuHCl was more effective than urea in denaturation of 11S. A two-step transition in 11S structure was observed with a possible existence of a folding intermediate at 2.5 M GuHCl. Sodium dodecyl sulfate (SDS) measurably altered secondary and tertiary structures of 11S and 7S below SDS critical micellar concentration (CMC), possibly due to formation of mixed peptide-SDS micelles. SDS treatment increased alpha-helical and unordered structures of both proteins at the expense of beta-sheet structure. NaCl and CaCl 2 caused a significant decrease in fluorescence intensity without shifting emission lambda max. Exposure of 7S and 11S to NaSCN respectively at >/=0.3 and >/=0.6 M NaSCN caused a significant increase in fluorescence intensity measured at the corresponding lambda max of the protein. beta-Mercaptoethanol (beta-ME), N-ethylmaleimide (NEM), and phytic acid caused variable red shifts, 2.5-4 nm, in the emission lambda max.

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Publishing Authors By Initials

KW Clara Sze,HH Kshirsagar,M Venkatachalam,SK Sathe,KW Clara Sze,HH Kshirsagar,M Venkatachalam,SK Sathe,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

PUBMED ID PMID:

MEDLINE DATE:

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of agricultural and food chemistry

VOLUME: 55

Page Numbers: 8745-53

Journal Abbreviation: J. Agric. Food Chem.

ISSN: 0021-8561

DAY: 20

MONTH: 09

YEAR: 2007

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374755

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S). Information

Substance Name: glycinin

Registry Number: 9007-93-6

Grant and Affiliation Information for A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S).

AFFILIATION: Department of Nutrition, Food & Exercise Sciences, College of Human Sciences, Florida State University, Tallahassee, Florida 32306-1493, USA. ssathe@mailer.fsu.edu

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Agric Food Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean Glycine max L storage proteins glycinin 11S and beta-conglycinin 7S Related Publications

• A circular dichroism and fluorescence spectrometric assessment of effects of selected chemical denaturants on soybean (Glycine max L.) storage proteins glycinin (11S) and beta-conglycinin (7S).
• Biochemical Composition and Immunological Comparison of Select Pecan [Carya illinoinensis (Wangenh.) K. Koch] Cultivars.
• Cardiac valvular inflammatory pseudotumor.
• Correlation between dark adaptation and refractive errors.
• Effects of processing on immunoreactivity of cashew nut (Anacardium occidentale L.) seed flour proteins.
• Enzyme immobilization on tritylagarose.
• Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Sulfur-Rich Protein (SRP) in Soybeans (Glycine max L.) and Certain Other Edible Plant Seeds.