A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme.

A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Research Abstract Details 

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A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Abstract Text:

S Takahashi,T Kontani,M Yoneda,T Ooi,

Disulfide-reduced RNase A, which could be reoxidized to give the native enzyme, was shown to have a CD spectrum quite different from that of the native enzyme or a random coil. Disulfide-reduced and fully cysteine-S-carboxamidomethylated RNase A because the derivative was stable and gave a spectrum identical to that of reduced RNase A. Curve-fitting analyses showed the presence of 14% alpha-helix and 25% beta-structure in this open chain derivative of RNase A. The time dependence of CD spectra during the oxidative renaturation of reduced RNase A was analyzed and changes in alpha-helical and beta-structure contents during the reaction were estimated. It was shown that the change in the content of beta-structure was slower than that of alpha-helix content and approximately paralleled the appearance of the enzymatic activity.

A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Publishing Authors By Initials

S Takahashi,T Kontani,M Yoneda,T Ooi,

For similar enzymes and coenzymes: enzymes: hydrolases: esterases: ribonucleases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: esterases: ribonucleases research

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A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 82

Page Numbers: 1127-33

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1977

A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Keywords Mesh Terms:

KEYWORDS: Ribonucleases

MESH TERMS: metabolism

Chemical & Substance for Abstract: A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme. Information

Substance Name: Ribonucleases

Registry Number: EC 3.1.-

Grant and Affiliation Information for A circular dichroic spectral study on disulfide-reduced pancreatic ribonuclease A and its renaturation to the active enzyme.

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Country: JAPAN

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MEDLINETA: J Biochem

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