A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing.

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Abstract Text:

Innokenti Toulokhonov,Jinwei Zhang,Murali Palangat,Robert Landick,

Transcriptional pausing by RNA polymerase is an underlying event in the regulation of transcript elongation, yet the physical changes in the transcribing complex that create the initially paused conformation remain poorly understood. We report that this nonbacktracked elemental pause results from an active-site rearrangement whose signature includes a trigger-loop conformation positioned near the RNA 3' nucleotide and a conformation of betaDloopII that allows fraying of the RNA 3' nucleotide away from the DNA template. During nucleotide addition, trigger-loop movements or folding appears to assist NTP-stimulated translocation and to be crucial for catalysis. At a pause, the trigger loop directly contributes to the paused conformation, apparently by restriction of its movement or folding, whereas a previously postulated unfolding of the bridge helix does not. This trigger-loop-centric model can explain many properties of transcriptional pausing.

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Publishing Authors By Initials

I Toulokhonov,J Zhang,M Palangat,R Landick,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular cell

VOLUME: 27

Page Numbers: 406-19

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 3

MONTH: Aug

YEAR: 2007

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing.

AFFILIATION: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 38660

ACRONYM: GM

MEDLINETA: Mol Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing Related Publications

• A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing.