A central role for SSB in Escherichia coli RecQ DNA helicase function.

A central role for SSB in Escherichia coli RecQ DNA helicase function. Research Abstract Details 

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A central role for SSB in Escherichia coli RecQ DNA helicase function. Abstract Text:

Robert D Shereda,Douglas A Bernstein,James L Keck,

RecQ DNA helicases are critical components of DNA replication, recombination, and repair machinery in all eukaryotes and bacteria. Eukaryotic RecQ helicases are known to associate with numerous genome maintenance proteins that modulate their cellular functions, but there is little information regarding protein complexes involving the prototypical bacterial RecQ proteins. Here we use an affinity purification scheme to identify three heterologous proteins that associate with Escherichia coli RecQ: SSB (single-stranded DNA-binding protein), exonuclease I, and RecJ exonuclease. The RecQ-SSB interaction is direct and is mediated by the RecQ winged helix subdomain and the C terminus of SSB. Interaction with SSB has important functional consequences for RecQ. SSB stimulates RecQ-mediated DNA unwinding, whereas deletion of the C-terminal RecQ-binding site from SSB produces a variant that blocks RecQ DNA binding and unwinding activities, suggesting that RecQ recognizes both the SSB C terminus and DNA in SSB.DNA nucleoprotein complexes. These findings, together with the noted interactions between human RecQ proteins and Replication Protein A, identify SSB as a broadly conserved RecQ-binding protein. These results also provide a simple model that explains RecQ integration into genome maintenance processes in E. coli through its association with SSB.

A central role for SSB in Escherichia coli RecQ DNA helicase function. Publishing Authors By Initials

RD Shereda,DA Bernstein,JL Keck,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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A central role for SSB in Escherichia coli RecQ DNA helicase function. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 19247-58

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 3

MONTH: 05

YEAR: 2007

A central role for SSB in Escherichia coli RecQ DNA helicase function. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

A central role for SSB in Escherichia coli RecQ DNA helicase function. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: A central role for SSB in Escherichia coli RecQ DNA helicase function. Information

Substance Name: RecQ protein, E coli

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for A central role for SSB in Escherichia coli RecQ DNA helicase function.

AFFILIATION: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706-1532, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM068061

ACRONYM: GM

MEDLINETA: J Biol Chem

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