A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium.

A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Research Abstract Details 

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A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Abstract Text:

Tong Gao,Celine Roisin-Bouffay,R Diane Hatton,Lei Tang,Debra A Brock,Tiffany DeShazo,Laura Olson,Wan-Pyo Hong,Wonhee Jang,Elvia Canseco,Deenadayalan Bakthavatsalam,Richard H Gomer,

Developing Dictyostelium cells form aggregation streams that break into groups of approximately 2 x 10(4) cells. The breakup and subsequent group size are regulated by a secreted multisubunit counting factor (CF). To elucidate how CF regulates group size, we isolated second-site suppressors of smlA(-), a transformant that forms small groups due to oversecretion of CF. smlA(-) sslA1(CR11) cells form roughly wild-type-size groups due to an insertion in the beginning of the coding region of sslA1, one of two highly similar genes encoding a novel protein. The insertion increases levels of SslA. In wild-type cells, the sslA1(CR11) mutation forms abnormally large groups. Reducing SslA levels by antisense causes the formation of smaller groups. The sslA(CR11) mutation does not affect the extracellular accumulation of CF activity or the CF components countin and CF50, suggesting that SslA does not regulate CF secretion. However, CF represses levels of SslA. Wild-type cells starved in the presence of smlA(-) cells, recombinant countin, or recombinant CF50 form smaller groups, whereas sslA1(CR11) cells appear to be insensitive to the presence of smlA(-) cells, countin, or CF50, suggesting that the sslA1(CR11) insertion affects CF signal transduction. We previously found that CF reduces intracellular glucose levels. sslA(CR11) does not significantly affect glucose levels, while glucose increases SslA levels. Together, the data suggest that SslA is a novel protein involved in part of a signal transduction pathway regulating group size.

A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Publishing Authors By Initials

T Gao,C Roisin-Bouffay,RD Hatton,L Tang,DA Brock,T DeShazo,L Olson,WP Hong,W Jang,E Canseco,D Bakthavatsalam,RH Gomer,

For similar genetic processes: mutagenesis: suppression, genetic research abstracts see: genetic processes: mutagenesis: suppression, genetic research

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A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Eukaryotic cell

VOLUME: 6

Page Numbers: 1538-51

Journal Abbreviation: Eukaryotic Cell

ISSN: 1535-9778

DAY: 27

MONTH: 07

YEAR: 2007

A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Information

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LANGUAGE: eng

NlmUniqueID: 101130731

A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Keywords Mesh Terms:

KEYWORDS: Suppression, Genetic

MESH TERMS: pharmacology

Chemical & Substance for Abstract: A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium. Information

Substance Name: Cyclic AMP

Registry Number: 60-92-4

Grant and Affiliation Information for A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium.

AFFILIATION: Howard Hughes Medical Institute, MS-140, Rice University, 6100 S. Main Street, Houston, TX 77005-1892, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 074990

ACRONYM: GM

MEDLINETA: Eukaryot Cell

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