A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae.

A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Research Abstract Details 

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A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Abstract Text:

David J Adle,Devis Sinani,Heejeong Kim,Jaekwon Lee,

Detoxification and homeostatic acquisition of metal ions are vital for all living organisms. We have identified PCA1 in yeast Saccharomyces cerevisiae as an overexpression suppressor of copper toxicity. PCA1 possesses signatures of a P1B-type heavy metal-transporting ATPase that is widely distributed from bacteria to humans. Copper resistance conferred by PCA1 is not dependent on catalytic activity, but it appears that a cysteine-rich region located in the N terminus sequesters copper. Unexpectedly, when compared with two independent natural isolates and an industrial S. cerevisiae strain, the PCA1 allele of the common laboratory strains we have examined possesses a missense mutation in a predicted ATP-binding residue conserved in P1B-type ATPases. Consistent with a previous report that identifies an equivalent mutation in a copper-transporting P1B-type ATPase of a Wilson disease patient, the PCA1 allele found in laboratory yeast strains is nonfunctional. Overexpression or deletion of the functional allele in yeast demonstrates that PCA1 is a cadmium efflux pump. Cadmium as well as copper and silver, but not other metals examined, dramatically increase PCA1 protein expression through post-transcriptional regulation and promote subcellular localization to the plasma membrane. Our study has revealed a novel metal detoxification mechanism in yeast mediated by a P1B-type ATPase that is unique in structure, substrate specificity, and mode of regulation.

A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Publishing Authors By Initials

DJ Adle,D Sinani,H Kim,J Lee,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 947-55

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 14

MONTH: 11

YEAR: 2006

A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Information

Substance Name: PCA1 protein, S cerevisiae

Registry Number: EC 3.6.3.4

Grant and Affiliation Information for A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae.

AFFILIATION: Redox Biology Center, Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR-17675

ACRONYM: RR

MEDLINETA: J Biol Chem

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