A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein.

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Abstract Text:

Hwan Youn,Robert L Kerby,Junseock Koh,Gary P Roberts,

The cAMP receptor protein (CRP) of Escherichia coli exists in an equilibrium between active and inactive forms, and the effector, cAMP, shifts that equilibrium to the active form, thereby allowing DNA binding. For this equilibrium shift, a C-helix repositioning around the C-helix residues Thr-127 and Ser-128 has been reported as a critical local event along with proper beta4/beta5 positioning. Here we show that another C-helix residue, Arg-123, has a unique role in cAMP-dependent CRP activation in two different ways. First, Arg-123 is important for proper cAMP affinity, although it is not critical for the conformational change with saturating amounts of cAMP. Second, Arg-123 is optimal for stabilizing the inactive conformation of CRP when cAMP is absent, thereby allowing a maximal range of regulation by cAMP. However, Arg-123 does not appear to be critical for a functional response to cAMP, as has been proposed previously (Berman, H. M., Ten Eyck, L. F., Goodsell, D. S., Haste, N. M., Korney, A., and Taylor, S. S. (2005) Proc. Natl. Acad. Sci. U. S. A. 102, 45-50). Based on mutagenic evidence, we also propose the basis for the stabilization of the inactive form to be through a salt interaction between Asp-68 and Arg-123.

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Publishing Authors By Initials

H Youn,RL Kerby,J Koh,GP Roberts,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

PUBMED ID PMID:

MEDLINE DATE:

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 3632-9

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 8

MONTH: 12

YEAR: 2006

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: physiology

Chemical & Substance for Abstract: A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Information

Substance Name: Arginine

Registry Number: 74-79-3

Grant and Affiliation Information for A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein.

AFFILIATION: Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM53228

ACRONYM: GM

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein Related Publications

• A pilot trial of plasma infusions in Duchenne muscular dystrophy.
• Acute effect of vasectomy on the function of the rat epididymal epithelium and vas deferens.
• Application of colorimetric assays to assess viability, growth and metabolism of hydrogel-encapsulated cells.
• CD47 is necessary for inhibition of nitric oxide-stimulated vascular cell responses by thrombospondin-1.
• Comparison of the antioxidant properties of alpha-tocopherol and alpha-tocopheryl acetate in newborn rabbit lung.
• Conceptus development, uterine response, blood gases and endocrine function of gilts exposed to increased ambient temperature during early pregnancy.
• Cost-effectiveness of low-molecular-weight heparin for secondary prophylaxis of cancer-related venous thromboembolism.
• Data-guided brain deformation modeling: evaluation of a 3-D adjoint inversion method in porcine studies.
• Deficiencies of cardiovascular risk prediction models for type 1 diabetes.
• Dementia and low testosterone and bioavailable testosterone levels in men: possible increased risk.
• Development of a real time quantitative PCR assay for the hard clam pathogen Quahog Parasite Unknown (QPX).
• Differential regulation of hepatic cytochrome P450 monooxygenases in streptozotocin-induced diabetic rats.
• Disposition of intravenously administered pharmacologic doses of vitamin E in newborn rabbits.
• Ethnic differences in adolescents' mental distress, social stress, and resources.
• Facts and ideas from anywhere.
• IRVING DAVID PRENGLER, MD, MBA: a conversation with the editor.
• Increasing the efficiency of Monte Carlo cohort simulations with variance reduction techniques.
• Origin and evolution of the TKDP gene family.
• PAUL BERNARD CONVERY, MD, MMM: a conversation with the editor.
• ROBERT GARY MENNEL, MD: a conversation with the editor.
• Relationship between doses of prostaglandin F(2alpha) and stages of the breeding season for synchronization of estrus and ovulation in ewes.
• Seasonal variation in gestational blood pressure.
• Sonic tooth brushing reduces gingival overgrowth in renal transplant recipients.
• Susceptibility of Escherichia coli to L-selenaproline and other L-proline analogues in laboratory culture media and normal human urine.
• Teaching medical decision modeling: a qualitative description of student errors and curriculum responses.
• The effect of age and role information on expectations for big five personality traits.
• The evolution of the art and science of theriogenology The Second David E. Bartlett Honorary Address.
• The occurrence of the cold-water coral Lophelia pertusa (Scleractinia) on oil and gas platforms in the North Sea: colony growth, recruitment and environmental controls on distribution.
• The p110alpha isoform of PI3K is essential for proper growth factor signaling and oncogenic transformation.
• Tissue vitamin E levels in newborn rabbits after pharmacologic dosing. Influence of dose, dosage form, and route of administration.