A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing.

A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Research Abstract Details 

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A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Abstract Text:

Sarah Yohannan,Salem Faham,Duan Yang,David Grosfeld,Aaron K Chamberlain,James U Bowie,

Hydrogen bonds involving a carbon donor are very common in protein structures, and energy calculations suggest that Calpha-H...O hydrogen bonds could be about one-half the strength of traditional hydrogen bonds. It has therefore been proposed that these nontraditional hydrogen bonds could be a significant factor in stabilizing proteins, particularly membrane proteins as there is a low dielectric and no competition from water in the bilayer core. Nevertheless, this proposition has never been tested experimentally. Here, we report an experimental test of the significance of Calpha-H...O bonds for protein stability. Thr24 in bacteriorhodopsin, which makes an interhelical Calpha-H...O hydrogen bond to the Calpha of Ala51, was changed to Ala, Val, and Ser, and the thermodynamic stability of the mutants was measured. None of the mutants had significantly reduced stability. In fact, T24A was more stable than the wild-type protein by 0.6 kcal/mol. Crystal structures were determined for each of the mutants, and, while some structural changes were seen for T24S and T24V, T24A showed essentially no apparent structural alteration that could account for the increased stability. Thus, Thr24 appears to destabilize the protein rather than stabilize. Our results suggest that Calpha-H...O bonds are not a major contributor to protein stability.

A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Publishing Authors By Initials

S Yohannan,S Faham,D Yang,D Grosfeld,AK Chamberlain,JU Bowie,

For similar natural sciences: physics: thermodynamics research abstracts see: natural sciences: physics: thermodynamics research

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A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 2284-5

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 3

MONTH: Mar

YEAR: 2004

A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Keywords Mesh Terms:

KEYWORDS: Thermodynamics

MESH TERMS: chemistry

Chemical & Substance for Abstract: A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Information

Substance Name: Bacteriorhodopsins

Registry Number: 53026-44-1

Grant and Affiliation Information for A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing.

AFFILIATION: Department of Chemistry and Biochemistry, UCLA-DOE Center for Genomics and Proteomics, Molecular Biology Institute, 655 Boyer Hall, University of California, Los Angeles, Los Angeles, California 90095-1570, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM63919

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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