A bichromatic fluorescent reporter for cell-based screens of alternative splicing.

A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Research Abstract Details 

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A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Abstract Text:

James P Orengo,Donnie Bundman,Thomas A Cooper,

Alternative splicing is the primary source of proteome complexity in metazoans and its regulation shapes the proteome in response to shifting physiological requirements. We developed a bichromatic splicing reporter that uses a peculiar feature of some fluorescent protein coding regions to express two different fluorescent proteins from a single alternative splicing event. The mutually exclusive expression of different fluorescent proteins from a single reporter provides a uniquely sensitive approach for high-throughput screening and analysis of cell-specific splicing events in mixed cell cultures and tissues of transgenic animals. This reporter is applicable to the majority of alternative splicing patterns and can be used to quantify alternative splicing within single cells and to select cells that express specific splicing patterns. The ability to perform quantitative single-cell analysis of alternative splicing and high-throughput screens will enhance progress toward understanding splicing regulatory networks and identifying compounds that reverse pathogenic splicing defects.

A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Publishing Authors By Initials

JP Orengo,D Bundman,TA Cooper,

For similar diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, fluorescence research abstracts see: diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, fluorescence research

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A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nucleic acids research

VOLUME: 34

Page Numbers: e148

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 16

MONTH: 11

YEAR: 2006

A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Keywords Mesh Terms:

KEYWORDS: Microscopy, Fluorescence

MESH TERMS: genetics

Chemical & Substance for Abstract: A bichromatic fluorescent reporter for cell-based screens of alternative splicing. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for A bichromatic fluorescent reporter for cell-based screens of alternative splicing.

AFFILIATION: Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.

Country: England

AGENCY: United States NHLBI

GRANT: R01HL45565

ACRONYM: HL

MEDLINETA: Nucleic Acids Res

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