A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties.

A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Research Abstract Details 

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A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Abstract Text:

R Tamura,Y Takesue,Y Nishi,

Actin and four major associated proteins have been identified in the microvillus cytoskeleton from chicken intestinal brush borders isolated in the presence of Ca2+-chelating agents. We have isolated microvilli from rabbit small intestine in the presence of Ca2+ and found by sodium dodecyl sulfate-gel electrophoresis that a protein of 33,000 daltons exists in a molar ratio to actin of 1:3 within the isolated microvillus. The protein could be extracted from acetone powder of microvilli in the absence, but not in the presence, of Ca2+. Thus, for its purification the acetone powder was extracted twice with buffer containing Ca2+ and then with buffer containing a Ca2+-chelator. The 33,000-dalton protein was purified from the last extract by Sephadex G-100 gel filtration and DEAE-cellulose column chromatography. The purified protein was a water-soluble monomeric protein with a molecular weight of 33,000. Antisera against the purified protein raised in guinea pigs reacted with the antigen but not with actin or tropomyosin purified from rabbit skeletal muscle. Freezing-thawing resulted in aggregation of the purified protein, and the aggregates showed filamentous and sheet-like structures. These results suggests that the 33,000-dalton protein is a major component of the microvillus cytoskeleton.

A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Publishing Authors By Initials

R Tamura,Y Takesue,Y Nishi,

For similar animals: chordata: vertebrates: mammals: lagomorpha: rabbits research abstracts see: animals: chordata: vertebrates: mammals: lagomorpha: rabbits research

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A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 92

Page Numbers: 1259-68

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1982

A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Keywords Mesh Terms:

KEYWORDS: Rabbits

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: A 33,000-dalton protein of the microvilli isolated from rabbit small-intestinal epithelial cells. Purification and some properties. Information

Substance Name: Calcium

Registry Number: 7440-70-2

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Country: JAPAN

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MEDLINETA: J Biochem

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