(99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents.

(99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents. Research Abstract Details 

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(99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents. Abstract Text:

I-Chien Wei,Ning Tsao,Ya-Hui Huang,Yen-Sheng Ho,Chung-Chin Wu,Dong-Fang Yu,David J Yang,

This study was aimed to develop a glycopeptide (GP) to be used as a carrier for anti-cancer drug delivery. GP was synthesized by conjugating glutamate peptide and chitosan using carbodiimide as a coupling agent. Elemental analysis and capillary electrophoresis confirmed the purity was >95%. GP was labeled with sodium pertechnetate (Na(99m)TcO(4)) for in vitro and in vivo studies. Rhenium-GP was synthesized to support the binding site of (99m)Tc at the glutamate positions 3-5. In vitro cellular uptake of (99m)Tc-GP was performed in breast cancer cells. Cytosol had 60% whereas nucleus had 40% uptake of (99m)Tc-GP. When cancer cells were incubated with glutamate or aspartate, followed by (99m)Tc-GP, there was decreased uptake in cells treated with glutamate but not aspartate. The findings indicated that cellular uptake of (99m)Tc-GP was via glutamate transporters. In addition, (99m)Tc-GP was able to measure uptake differences after cells treated with paclitaxel. Biodistribution and planar imaging were conducted in breast tumor-bearing rats. Biodistribution of (99m)Tc-GP showed increased tumor-to-tissue ratios as a function of time. Planar images confirmed that (99m)Tc-GP could assess tumor uptake changes after paclitaxel treatment. In vitro and in vivo studies indicated that GP could target tumor cells, thus, GP may be a useful carrier for anti-cancer drug delivery.

(99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents. Publishing Authors By Initials

IC Wei,N Tsao,YH Huang,YS Ho,CC Wu,DF Yu,DJ Yang,

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(99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Applied radiation and isotopes : including data, i

VOLUME: 66

Page Numbers: 320-31

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ISSN: 0969-8043

DAY: 17

MONTH: 10

YEAR: 2007

(99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents. Information

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LANGUAGE: eng

NlmUniqueID: 9306253

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Grant and Affiliation Information for (99m)Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents.

AFFILIATION: Center for Innovation Development, Taiwan Hopax Chems Mfg. Co., Ltd., No. 28, Hua Dong Road, Daliao, Kaohsiung 83162, Taiwan.

Country: England

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MEDLINETA: Appl Radiat Isot

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