3D reconstruction and processing of volumetric data in cryo-electron tomography.

3D reconstruction and processing of volumetric data in cryo-electron tomography. Research Abstract Details 

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3D reconstruction and processing of volumetric data in cryo-electron tomography. Abstract Text:

Hanspeter Winkler,

A software package is presented that was primarily developed for electron tomography in biological research. It comprises routines for preprocessing micrographs, CTF-correction of images of untilted and tilted specimens, alignment of tilt series, 3D reconstruction, spatial averaging of paracrystalline specimens, alignment of single particles or components of larger macromolecular assemblies, correlation averaging, multivariate statistical analysis, classification, and volume reassembly, in which molecular components in raw tomograms are replaced by averaged versions with higher signal-to-noise ratio. The emphasis for image analysis is placed on the processing of large sets of individual molecular volumes. The design philosophy of the software is both simplicity and interoperability, i.e. to write standalone programs for simple tasks that are combined through shell scripting to provide more complex functionality, and to communicate with other software via common image formats. This paper gives an overview of the current state of the programs and some applications to cryo-electron tomography.

3D reconstruction and processing of volumetric data in cryo-electron tomography. Publishing Authors By Initials

H Winkler,

For similar information science: computing methodologies: software research abstracts see: information science: computing methodologies: software research

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3D reconstruction and processing of volumetric data in cryo-electron tomography. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of structural biology

VOLUME: 157

Page Numbers: 126-37

Journal Abbreviation: J. Struct. Biol.

ISSN: 1047-8477

DAY: 11

MONTH: 08

YEAR: 2006

3D reconstruction and processing of volumetric data in cryo-electron tomography. Information

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LANGUAGE: eng

NlmUniqueID: 9011206

3D reconstruction and processing of volumetric data in cryo-electron tomography. Keywords Mesh Terms:

KEYWORDS: Software

MESH TERMS: methods

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Grant and Affiliation Information for 3D reconstruction and processing of volumetric data in cryo-electron tomography.

AFFILIATION: Florida State University, Institute of Molecular Biophysics, Tallahassee, FL 32306, USA. winkler@sb.fsu.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM64346

ACRONYM: GM

MEDLINETA: J Struct Biol

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