3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics.

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Abstract Text:

BACKGROUND: The in vivo hematopoietic microenvironment is composed of stromal cells and extracellular matrix in a 3D configuration. We have created a 3D microenvironment in vitro, employing spatial development of stromal cells in a nonwoven fabric porous carrier, Fibra-cel (FC). We compared its performance with that of a 2D microenvironment. METHODS: Primary murine BM cells were inoculated on the layers of stromal cells prepared in FC (3D) or on a dish (2D) and cultured for 7-21 days. The hematopoietic cells harvested from the cultures were evaluated by colony-forming unit (CFU) assay and transplantation to sub lethally irradiated mice. RESULTS: The maximum stromal cell concentration in the 2D culture was higher than that in the 3D culture. However, the hematopoietic cell concentration in the 3D culture was kept at a higher level than that in the 2D culture. The number of CFU-mix increased five times during 3D cultivation, but decreased in the 2D culture. The engraftment percentage of 3D cultured cells was comparable with that of fresh cells, and markedly higher than that of 2D cultured cells. DISCUSSION: The 3D culture constructed with FC and stromal cells was clearly superior to 2D culture because hematopoietic progenitor cells were expanded without the addition of cytokines and the content of hematopoietic stem cells was maintained.

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Publishing Authors By Initials

For similar cells: connective tissue cells: stromal cells research abstracts see: cells: connective tissue cells: stromal cells research

PUBMED ID PMID:

MEDLINE DATE:

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cytotherapy

VOLUME: 4

Page Numbers: 285-91

Journal Abbreviation: Cytotherapy

ISSN: 1465-3249

DAY: 24

MONTH: 04

YEAR: 2002

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100895309

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Keywords Mesh Terms:

KEYWORDS: Stromal Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: 3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics. Information

Substance Name: Biocompatible Materials

Registry Number: 0

Grant and Affiliation Information for 3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics.

AFFILIATION: International Center for Biotechnology, Osaka Univeristy, Suita, Osaka, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cytotherapy

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics Related Publications

• "Watermelon rectum" associated with multiple vascular ectasia in the colon.
• A compact UHV X-ray diffractometer for studying surfaces and interfaces.
• Abnormal course of the oculomotor nerve on the clivus combined with a petroclival meningioma: case report.
• Application of independent component analysis and expansion matching filter for automatic detection of motor unit action potential trains.
• Application of near-infrared spectroscopy to measuring of attractiveness of opposite-sex faces.
• Binding sites on laminin receptors as components for antibiotics.
• CYP17 polymorphism and tamoxifen-induced hepatic steatosis.
• Chromosomal abnormalities subdivide neuroepithelial tumors into clinically relevant groups.
• Clear cell hidradenoma of the breast: a case report with review of the literature.
• Clinical characteristics of hypotonia: a survey of pediatric physical and occupational therapists.
• Detection of high concentrations of 137Cs in Walleye pollock collected in the Sea of Japan.
• Efficacy of recombinant annexin 2 for fibrinolytic therapy in a rat embolic stroke model: a magnetic resonance imaging study.
• Electrochemical generation of glycosyl triflate pools.
• Electron microscopic examination of the dormant spore and the sporulation of Paenibacillus motobuensis strain MC10.
• Experimental and analytical investigations of motor unit location for the precise estimation of muscle force with surface electromyograms.
• F1-ATPase rotates by an asymmetric, sequential mechanism using all three catalytic subunits.
• Feasibility of oxaliplatin and infusional fluorouracil/leucovorin (FOLFOX4) for Japanese patients with unresectable metastatic colorectal cancer.
• Fully Automated Three-Dimensional Detection of Polyps in Fecal-Tagging CT Colonography.
• Iatrogenic perforation associated with therapeutic colonoscopy: a multicenter study in Japan.
• Insufficient outcomes with imatinib mesylate: case report of Ph-positive acute myeloid leukemia evolving from myelodysplastic syndrome.
• Interferential current stimulation for sensory communication between prosthetic hand and man.
• Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury.
• Photoactivated ethidium monoazide directly cleaves bacterial DNA and is applied to PCR for discrimination of live and dead bacteria.
• Protein kinase C delta activates IkappaB-kinase alpha to induce the p53 tumor suppressor in response to oxidative stress.
• Recovery of olfactory function after an anterior craniofacial approach.
• Retrospective review of pediatric and adult autoimmune hepatitis in two quaternary care centres in British Columbia: increased prevalence seen in British Columbia's First Nations community.
• STUDIES ON THE EXPRESSION LEVELS OF STEROL-METABOLIZING ENZYMES IN THE OBESE MODEL SHR/NDmcr-cp RATS.
• Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA.
• Surface modification of poly(ethylene terephthalate) by plasma polymerization of poly(ethylene glycol).
• Varicella zoster virus meningoencephalitis accompanied by sporadic skin lesions in an older immunocompetent adult.