23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22.

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Abstract Text:

Rita Berisio,Natascia Corti,Peter Pfister,Ada Yonath,Erik C ,

Resistance to macrolides and ketolides occurs mainly via alterations in RNA moieties of the drug-binding site. Using an A2058G mutant of Mycobacterium smegmatis, additional telithromycin resistance was acquired via deletion of 15 residues from protein L22. Molecular modeling, based on the crystal structure of the large ribosomal subunit from Deinococcus radiodurans complexed with telithromycin, shows that the telithromycin carbamate group is located in the proximity of the tip of the L22 hairpin-loop, allowing for weak interactions between them. These weak interactions may become more important once the loss of A2058 interactions destabilizes drug binding, presumably resulting in a shift of the drug toward the other side of the tunnel, namely, to the vicinity of L22. Hence, the deletion of 15 residues from L22 may further destabilize telithromycin binding and confer telithromycin resistance. Such deletions may also lead to notable differences in the tunnel outline, as well as to an increase of its diameter to a size, allowing the progression of the nascent chain.

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Publishing Authors By Initials

R Berisio,N Corti,P Pfister,A Yonath,EC ,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research

PUBMED ID PMID:

MEDLINE DATE:

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Antimicrobial agents and chemotherapy

VOLUME: 50

Page Numbers: 3816-23

Journal Abbreviation: Antimicrob. Agents Chemother.

ISSN: 0066-4804

DAY: 21

MONTH: 08

YEAR: 2006

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 315061

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Keywords Mesh Terms:

KEYWORDS: Structure-Activity Relationship

MESH TERMS: physiology

Chemical & Substance for Abstract: 23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22. Information

Substance Name: telithromycin

Registry Number: 0

Grant and Affiliation Information for 23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22.

AFFILIATION: Department for Structural Biology, Weizmann Institute of Science, Rehovot, Israel. ada.yonath@weizmann.ac.il

Country: United States

AGENCY: United States NIGMS

GRANT: GM34360

ACRONYM: GM

MEDLINETA: Antimicrob Agents Chemother

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22 Related Publications

• 23S rRNA 2058A-->G alteration mediates ketolide resistance in combination with deletion in L22.
• A naturally occurring cancer with molecular connectivity to human diseases.
• Conservation of bacterial protein synthesis machinery: initiation and elongation in Mycobacterium smegmatis.
• Measurement of the fluence response of the GSI neutron ball dosemeter in the energy range from thermal to 19 MeV.
• Minimal thyroid cancer: clinical consequences.
• Mitochondrial deafness alleles confer misreading of the genetic code.
• [Champfer-shoulder-pin attachment (III)]
• [Champfer-shoulder-pin attachment (conclusion)]
• [Mycologic studies on the problem of endangering the newborn infant through saccharomycetic flora in the mother]
• [Pancreatic tumor of uncertain origin--a therapeutic dilemma?]
• [Rational diagnosis in diseases of the esophagus and stomach]
• [The glide plane directed lead with the Gnathomat articulator]