1H NMR study on amide proton exchange of calmodulin-mastoparan complex.

1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Research Abstract Details 

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1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Abstract Text:

S Ohki,S Tsuda,S Joko,M Yazawa,K Yagi,K Hikichi,

Amide proton exchange rates of Ca2(+)-saturated calmodulin and Ca2(+)-saturated calmodulin-mastoparan complex were studied by 1H NMR spectroscopy. Exchange rates of Gly25, Gly61, Gly98, Gly134, Ile27, Ile100, and Asn137 were determined for Ca2(+)-saturated calmodulin and for Ca2(+)-saturated calmodulin-mastoparan complex, and were found to be less than 10(-4)s-1. All these residues of which the amide proton resonances appear at lower fields were considered to form hydrogen bonds, based on the results of X-ray analysis. Exchange rates of Ile27 and Asn137 became an order of magnitude smaller when mastoparan bound to Ca2(+)-saturated calmodulin, while those of the four glycines and Ile100 did not change appreciably. The reduction in accessibility of Asn137 to water cased by mastoparan binding suggests that a part of the mastoparan binding site is probably located in or near the hydrophobic cluster of the C-terminal-half domain. The reduction in accessibility of Ile27 also suggests that another part of the mastoparan binding site is located in or near the hydrophobic cleft of the N-terminal-half domain.

1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Publishing Authors By Initials

S Ohki,S Tsuda,S Joko,M Yazawa,K Yagi,K Hikichi,

For similar investigative techniques: chemistry, analytical: crystallography: x-ray diffraction research abstracts see: investigative techniques: chemistry, analytical: crystallography: x-ray diffraction research

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1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 109

Page Numbers: 234-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1991

1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Information

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LANGUAGE: eng

NlmUniqueID: 376600

1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Keywords Mesh Terms:

KEYWORDS: X-Ray Diffraction

MESH TERMS: metabolism

Chemical & Substance for Abstract: 1H NMR study on amide proton exchange of calmodulin-mastoparan complex. Information

Substance Name: Water

Registry Number: 7732-18-5

Grant and Affiliation Information for 1H NMR study on amide proton exchange of calmodulin-mastoparan complex.

AFFILIATION: Department of Polymer Science, Faculty of Science, Hokkaido University.

Country: JAPAN

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MEDLINETA: J Biochem

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