1H-1H dipolar couplings provide a unique probe of RNA backbone structure.

1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Research Abstract Details 

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1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Abstract Text:

Emeric Miclet,Erin O'Neil-Cabello,Edward P Nikonowicz,David Live,Ad Bax,

A NMR method is described that permits simultaneous measurement of the geminal 2JH1H2 + 2DH1H2 splitting and the sum of the 1JCH1 + 1DCH1 + 1JCH2 + 1DCH2 couplings for methylene groups, where 2DH1H2 and 1DCH are residual dipolar couplings, occurring when molecules are weakly oriented relative to the magnetic field. By suppressing either the upfield or downfield half of the 1H-1H geminal doublet, the experiment yields improved resolution relative to regular two-dimensional 1H-13C correlation spectra, making it applicable to systems of considerable complexity. The method is demonstrated for measurement of all 2DH5'H5'' couplings in a 24-nucleotide 13C-enriched RNA stem loop structure, weakly aligned in liquid crystalline Pf1. The method is equally applicable to methylene groups in 13C-labeled proteins and to natural abundance samples of smaller molecules.

1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Publishing Authors By Initials

E Miclet,E O'Neil-Cabello,EP Nikonowicz,D Live,A Bax,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, ribosomal research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, ribosomal research

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1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 15740-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 24

MONTH: Dec

YEAR: 2003

1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Keywords Mesh Terms:

KEYWORDS: RNA, Ribosomal

MESH TERMS: chemistry

Chemical & Substance for Abstract: 1H-1H dipolar couplings provide a unique probe of RNA backbone structure. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for 1H-1H dipolar couplings provide a unique probe of RNA backbone structure.

AFFILIATION: Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0520, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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