19F-NMR reveals metal and operator-induced allostery in MerR.

19F-NMR reveals metal and operator-induced allostery in MerR. Research Abstract Details 

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19F-NMR reveals metal and operator-induced allostery in MerR. Abstract Text:

Lingyun Song,Quincy Teng,Robert S Phillips,John M Brewer,Anne O Summers,

Metalloregulators of the MerR family activate transcription upon metal binding by underwinding the operator-promoter DNA to permit open complex formation by pre-bound RNA polymerase. Historically, MerR's allostery has been monitored only indirectly via nuclease sensitivity or by fluorescent nucleotide probes and was very specific for Hg(II), although purified MerR binds several thiophilic metals. To observe directly MerR's ligand-induced behavior we made 2-fluorotyrosine-substituted MerR and found similar, minor changes in (19)F chemical shifts of tyrosine residues in the free protein exposed to Hg(II), Cd(II) or Zn(II). However, DNA binding elicits large chemical shift changes in MerR's tyrosine residues and in DNA-bound MerR Hg(II) provokes changes very distinct from those of Cd(II) or Zn(II). These chemical shift changes and other biophysical and phenotypic properties of wild-type MerR and relevant mutants reveal elements of an allosteric network that enables the coordination state of the metal binding site to direct metal-specific movements in the distant DNA binding site and the DNA-bound state also to affect the metal binding domain.

19F-NMR reveals metal and operator-induced allostery in MerR. Publishing Authors By Initials

L Song,Q Teng,RS Phillips,JM Brewer,AO Summers,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

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19F-NMR reveals metal and operator-induced allostery in MerR. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of molecular biology

VOLUME: 371

Page Numbers: 79-92

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 10

MONTH: 05

YEAR: 2007

19F-NMR reveals metal and operator-induced allostery in MerR. Information

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LANGUAGE: eng

NlmUniqueID: 2985088

19F-NMR reveals metal and operator-induced allostery in MerR. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: metabolism

Chemical & Substance for Abstract: 19F-NMR reveals metal and operator-induced allostery in MerR. Information

Substance Name: 2-fluorotyrosine

Registry Number: 7656-31-7

Grant and Affiliation Information for 19F-NMR reveals metal and operator-induced allostery in MerR.

AFFILIATION: Department of Microbiology, University of Georgia, Athens, GA 30602, USA.

Country: England

AGENCY: United States PHS

GRANT: ER64108

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MEDLINETA: J Mol Biol

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