15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions.

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Abstract Text:

Xuanjun Ai,Zheng Zhou,Yawen Bai,Wing-Yiu Choy,

The effects of macromolecular crowding on protein stability and folding kinetics have been studied using the recently developed 15N spin relaxation dispersion technique. By applying this method to a redesigned apocytochrome b562, the kinetics and thermodynamics of the protein folding processes in both the presence and the absence of crowding agents have been characterized. The result indicates that, even under the mild crowded environments (in the presence of 85 mg/mL of PEG 20K), the folding rate of the protein can speed up significantly while the unfolded rate remains unchanged within experimental error.

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Publishing Authors By Initials

X Ai,Z Zhou,Y Bai,WY Choy,

For similar natural sciences: physics: thermodynamics research abstracts see: natural sciences: physics: thermodynamics research

PUBMED ID PMID:

MEDLINE DATE:

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 3916-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 29

MONTH: Mar

YEAR: 2006

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Keywords Mesh Terms:

KEYWORDS: Thermodynamics

MESH TERMS: methods

Chemical & Substance for Abstract: 15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions. Information

Substance Name: Nitrogen Isotopes

Registry Number: 0

Grant and Affiliation Information for 15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions.

AFFILIATION: Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions Related Publications

• 'Stockbroker's knee'.
• 15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions.
• A new protocol for high-yield purification of recombinant human prothymosin alpha expressed in Escherichia coli for NMR studies.
• A new repository penicillin (a form of aqueous penicillin G procaine) in infants and children.
• A phase I trial of outpatient weekly irinotecan/carboplatin and concurrent radiation for stage III unresectable non small-cell lung cancer: a Vanderbilt-Ingram Cancer Center Affiliate Network Trial.
• Anticancer drug-inorganic nanohybrid and its cellular interaction.
• Cellular toxicity of inorganic hydroxide nanoparticles.
• Changes in activity level in women aged 40-80 years.
• Differential effects of dietary vitamin E and antioxidants on eicosanoid synthesis in young rabbits.
• Dry eye and blink rate simulation with a pig eye model.
• EULAR evidence-based recommendations for the management of fibromyalgia syndrome.
• Effect of copper doping on the crystal structure and morphology of 1D nanostructured manganese oxides.
• Enhanced contrast of electrochromic full cell systems with nanocrystalline PEDOT-prussian blue.
• Hidden charge 2e Boson in doped Mott insulators.
• INDUCTION OF INCREASED FIBRINOLYTIC ACTIVATOR IN PLASMA BY VARIOUS DRUGS.
• Joint inflammation and cytokine inhibition in rheumatoid arthritis.
• Keratoacanthoma and infundibulocystic squamous cell carcinoma.
• Lead zirconate titanate/poly(vinylidene fluoride-trifluoroethylene) 1-3 composites for ultrasonic transducer applications.
• Mixed micelle-template route to mesoporous silica.
• Noninvasive Real-time Imaging of Tumors and Metastases Using Tumor-targeting Light-emitting Escherichia coli.
• Piezoelectric and pyroelectric properties of PZT/P(VDF-TrFE) composites with constituent phases poled in parallel or antiparallel directions.
• Point injection as an alternative acupuncture technique - an exploratory study of responses in healthy subjects.
• Potential for combined modality therapy of cyclooxygenase inhibitors and radiation.
• Sciatic pain.
• Soft-chemical synthesis and electrochemical characterization of multicomponent Mn(1-x-y)Co(x)Ni(y)O2 nanostructures.
• Synthesis of new visible light active photocatalysts of Ba(In(1/3)Pb(1/3)M'(1/3))O3 (M' = Nb, Ta): a band gap engineering strategy based on electronegativity of a metal component.
• Test characteristics of a level I or II fetal ultrasound in detecting structural heart disease.
• The benefits of in-home pharmacy evaluation for older persons.
• The intrinsically disordered TC-1 interacts with Chibby via regions with high helical propensity.
• Time-dependent X-ray absorption spectroscopic (XAS) study on the transformation of zinc basic salt into bis(N-oxopyridine-2-thionato) zinc (II).