15N metabolic labeling of mammalian tissue with slow protein turnover.

15N metabolic labeling of mammalian tissue with slow protein turnover. Research Abstract Details 

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15N metabolic labeling of mammalian tissue with slow protein turnover. Abstract Text:

Daniel B McClatchy,Meng-Qiu Dong,Christine C Wu,John D Venable,John R Yates,

We previously reported the metabolic 15N labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% 15N enrichment throughout all tissues of the rat. A high 15N enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of disease targeting any tissue.

15N metabolic labeling of mammalian tissue with slow protein turnover. Publishing Authors By Initials

DB McClatchy,MQ Dong,CC Wu,JD Venable,JR Yates,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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15N metabolic labeling of mammalian tissue with slow protein turnover. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of proteome research

VOLUME: 6

Page Numbers: 2005-10

Journal Abbreviation:

ISSN: 1535-3893

DAY: 22

MONTH: 03

YEAR: 2007

15N metabolic labeling of mammalian tissue with slow protein turnover. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101128775

15N metabolic labeling of mammalian tissue with slow protein turnover. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: methods

Chemical & Substance for Abstract: 15N metabolic labeling of mammalian tissue with slow protein turnover. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for 15N metabolic labeling of mammalian tissue with slow protein turnover.

AFFILIATION: Department of Cell Biology, 10550 North Torrey Pines Road, SR11, The Scripps Research Institute, LaJolla, California 92037, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41 RR11823-10

ACRONYM: RR

MEDLINETA: J Proteome Res

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