15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation.

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Abstract Text:

Rose M Rizek,Carole S Watson,Sarah Keating,Hsin-Hsiung Tai,John R G Challis,Alan D Bocking,

Prostaglandins play a central role in the stimulation and maintenance of both term and preterm labor. 15-Hydroxyprostaglandin dehydrogenase (PGDH), localized primarily to chorion trophoblasts, is the key enzyme responsible for the metabolism of prostaglandins. In preterm chorion, levels of PGDH protein and activity were lower when compared to term and were further reduced with the presence of infection, but effects of subclinical inflammation and membrane rupture on PGDH expression are not known. Our objectives were (1) to determine the relative expression of PGDH in amnion and chorion and (2) to determine the effect of preterm premature rupture of membranes (PPROM) and (3) subclinical inflammation on PGDH protein expression in preterm fetal membranes. Fetal membranes were collected from women with idiopathic preterm labor. Patients were divided into preterm birth (1) <32 weeks with PPROM (n = 6), (2) <32 weeks with intact membranes (n = 11), (3) >or=32 and <37 weeks with PPROM (n = 10), and (4) >or=32 and <37 weeks with intact membranes (n = 10). Different antibodies were used to detect protein expression and localization of PGDH in amnion and chorion from these patients using both Western blotting and immunohistochemistry. Antibody T (AbT) localized PGDH to chorion trophoblasts, whereas antibody C (AbC) detected immunoreactive (ir) PGDH predominantly in the amnion mesenchyme. By Western blot, AbT showed a stronger 29-kDa ir-PGDH band whereas with AbC, a stronger 55-kDa ir-PGDH signal was detected. 55-kDa ir-PGDH was significantly higher in PPROM amnion, specifically in the <32 weeks group (P < .05) and with PPROM >24 hours (P < .05). No change was detected in the 29-kDa ir-PGDH in either amnion or chorion with gestational age or the presence and absence of PPROM. In addition, neither form of ir-PGDH was altered significantly with or without subclinical inflammation. ir-PGDH is detectable in both chorion trophoblasts and amnion, especially in the mesenchyme; however, the predominant form of the enzyme differs in the 2 tissues. PPROM and subclinical inflammation do not appear to affect the levels of 29-kDa ir-PGDH protein in the fetal membranes. The differential expression of 55-kDa ir-PGDH in preterm amnion with and without PPROM supports the need for a better understanding of the different forms of PGDH.

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Publishing Authors By Initials

RM Rizek,CS Watson,S Keating,HH Tai,JR Challis,AD Bocking,

For similar cells: trophoblasts research abstracts see: cells: trophoblasts research

PUBMED ID PMID:

MEDLINE DATE:

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Reproductive sciences (Thousand Oaks, Calif.)

VOLUME: 14

Page Numbers: 260-9

Journal Abbreviation: Reprod Sci

ISSN: 1933-7205

DAY: 21

MONTH: Apr

YEAR: 2007

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101291249

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Keywords Mesh Terms:

KEYWORDS: Trophoblasts

MESH TERMS: pathology

Chemical & Substance for Abstract: 15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation. Information

Substance Name: 15-hydroxyprostaglandin dehydrogenase

Registry Number: EC 1.1.1.141

Grant and Affiliation Information for 15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation.

AFFILIATION: Department of Obstetrics and Gynecology, University of Toronto and Mount Sinai Hospital, Toronto, Ontario, Canada.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Reprod Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation Related Publications

• 15-Hydroxyprostaglandin dehydrogenase protein expression in human fetal membranes with and without subclinical inflammation.
• Prediction of preterm birth in symptomatic women using decision tree modeling for biomarkers.