14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking.

14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Research Abstract Details 

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14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Abstract Text:

Juan Rong,Shihua Li,Guoqing Sheng,Meng Wu,Brian Coblitz,Min Li,Haian Fu,Xiao-Jiang Li,

HAP1 (Huntingtin-associated protein 1) consists of two alternately spliced isoforms (HAP1A and HAP1B, which have unique C-terminal sequences) and participates in intracellular trafficking. The C terminus of HAP1A is phosphorylated, and this phosphorylation was found to decrease the association of HAP1A with kinesin light chain, a protein involved in anterograde transport in cells. It remains unclear how this phosphorylation functions to regulate the association of HAP1 with trafficking proteins. Using the yeast two-hybrid system, we found that HAP1 also interacts with 14-3-3 proteins, which are involved in the assembly of protein complexes and the regulation of protein trafficking. The interaction of HAP1 with 14-3-3 is confirmed by their immunoprecipitation and colocalization in mouse brain. Moreover, this interaction is specific to HAP1A and is increased by the phosphorylation of the C terminus of HAP1A. We also found that expression of 14-3-3 decreases the association of HAP1A with kinesin light chain. As a result, there is less HAP1A distributed in neurite tips of PC12 cells that overexpress 14-3-3. Also, overexpression of 14-3-3 reduces the effect of HAP1A in promoting neurite outgrowth of PC12 cells. We propose that the phosphorylation-dependent interaction of HAP1A with 14-3-3 regulates HAP1 function by influencing its association with kinesin light chain and trafficking in neuronal processes.

14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Publishing Authors By Initials

J Rong,S Li,G Sheng,M Wu,B Coblitz,M Li,H Fu,XJ Li,

For similar investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research abstracts see: investigative techniques: genetic techniques: cloning, molecular: two-hybrid system techniques research

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MEDLINE DATE:

14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 4748-56

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 13

MONTH: 12

YEAR: 2006

14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Keywords Mesh Terms:

KEYWORDS: Two-Hybrid System Techniques

MESH TERMS: physiology

Chemical & Substance for Abstract: 14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking. Information

Substance Name: kinesin light-chain proteins

Registry Number: 0

Grant and Affiliation Information for 14-3-3 protein interacts with Huntingtin-associated protein 1 and regulates its trafficking.

AFFILIATION: Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS36323

ACRONYM: NS

MEDLINETA: J Biol Chem

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