13C-13C NOESY: an attractive alternative for studying large macromolecules.

13C-13C NOESY: an attractive alternative for studying large macromolecules. Research Abstract Details 

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13C-13C NOESY: an attractive alternative for studying large macromolecules. Abstract Text:

Ivano Bertini,Isabella C Felli,Rainer ,Detlef Moskau,Roberta Pierattelli,

13C direct detection provides a valuable alternative to 1H detection to overcome fast relaxation because of its smaller magnetic moment. 13C-13C NOESY spectra were acquired for a dimeric protein of molecular mass 32 000 and for a monomeric analogue. With increasing molecular mass, the quality of 13C-13C NOESY spectra improves while the scalar-based experiments become less sensitive, as predicted by the increase in the molecular mass. 13C-13C NOESY spectra of the dimer were acquired with different mixing times. The mixing time can be tuned to detect mainly one-bond correlations, or it can be increased to also detect correlations between nuclei at longer distances. It is proposed that 13C-13C dipolar-based experiments provide a promising tool for signal detection and assignment in large macromolecules, such as multimeric species and macromolecular complexes, for which scalar-based experiments become less effective.

13C-13C NOESY: an attractive alternative for studying large macromolecules. Publishing Authors By Initials

I Bertini,IC Felli,R ,D Moskau,R Pierattelli,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

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13C-13C NOESY: an attractive alternative for studying large macromolecules. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 464-5

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 21

MONTH: Jan

YEAR: 2004

13C-13C NOESY: an attractive alternative for studying large macromolecules. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

13C-13C NOESY: an attractive alternative for studying large macromolecules. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: chemistry

Chemical & Substance for Abstract: 13C-13C NOESY: an attractive alternative for studying large macromolecules. Information

Substance Name: Superoxide Dismutase

Registry Number: EC 1.15.1.1

Grant and Affiliation Information for 13C-13C NOESY: an attractive alternative for studying large macromolecules.

AFFILIATION: CERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Florence, Italy. bertini@cerm.unifi.it

Country: United States

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MEDLINETA: J Am Chem Soc

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