1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland.

1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Research Abstract Details 

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1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Abstract Text:

S H Park,K Kano,Y Seyama,

Using a primary culture system of guinea pig Harderian gland cells, we investigated the metabolism of a unique lipid: 1-alkyl-2,3-diacylglycerol containing methyl-branched fatty acids. The cells were obtained by collagenase digestion, and cells with lipid-droplets were collected by two-step centrifugation. We cultured these cells, and examined their lipid and fatty acid compositions. The de novo synthesis of lipids in these cells was studied as to the incorporation of [1(2)-14C]acetate and [U-14C]glucose. The major lipid proved to be 1-alkyl-2,3-diacylglycerol, as in tissue, and it contained a large amount of methylbranched fatty acids specific to this gland. The incorporation of [14C]acetate and [14C]glucose into 1-alkyl-2,3-diacylglycerol in the cultured cells amounted to 79.7 and 88.2% of the total incorporation into the lipid fraction, respectively. The incorporation of [14C]acetate into fatty acids in the cultured cells was detected for the chain lengths of C14 to C25. The activities of glycerol-3-phosphate dehydrogenase in the cultured cells and Harderian gland were lower than that in adipose tissue. These results confirm that cultured cells reflect the lipid metabolism originating in the Harderian gland and show that this culture system can serve as one part of the armamentarium for further study of this unique lipid metabolism.

1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Publishing Authors By Initials

SH Park,K Kano,Y Seyama,

For similar animal structures: harderian gland research abstracts see: animal structures: harderian gland research

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1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 114

Page Numbers: 492-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1993

1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Information

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LANGUAGE: eng

NlmUniqueID: 376600

1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Keywords Mesh Terms:

KEYWORDS: Harderian Gland

MESH TERMS: metabolism

Chemical & Substance for Abstract: 1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland. Information

Substance Name: Collagenases

Registry Number: EC 3.4.24.-

Grant and Affiliation Information for 1-Alkyl-2,3-diacylglycerol synthesis in primary culture cells of guinea pig harderian gland.

AFFILIATION: Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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