Yeast Protein Protocols

Protocols and information related to yeast proteins. Including: Yeast Immunoprecipitation; Yeast Protein Purification and Isolation; Yeast Protein-Protein Interactions.

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Yeast Protein Protocols Categories

Yeast Immunoprecipitation Protocols (4)

Yeast Protein Protein Interactions Protocols (4)

Yeast Protein Purification and Isolation Protocols (5)

Yeast Protein Protocols Protocol Links Sort by: Hits | Alphabetical

Detection of Ubiquitylated Proteins in Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4615

This assay is performed to detect ubiquitylated proteins in yeast. Yeast that have been transformed with a vector expressing polyhistidine-tagged ubiquitin (Ub) under the control of a copper-inducible promoter are grown, induced with copper, and harvested. Total ubiquitylated proteins are then recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting. - [Read Detection of Ubiquitylated Proteins in Yeast Protocol]

Pulse-Chase Assay for Measuring Protein Stability in Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4641

This assay directly measures the stability of a specific protein in yeast. Following pulse-labeling of yeast cells, the target protein is recovered by denaturing immunoprecipitation. - [Read Pulse-Chase Assay for Measuring Protein Stability in Yeast Protocol]

Yeast Protein Extracts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4152

Protocol is a simple, reliable method for the preparation of yeast protein extracts for analysis by polyacrylamide gel electrophoresis (PAGE) and Western blotting. - [Read Yeast Protein Extracts Protocol]