Protocols and information related to yeast proteins. Including: Yeast Immunoprecipitation; Yeast Protein Purification and Isolation; Yeast Protein-Protein Interactions.
Detection of Ubiquitylated Proteins in Yeast Protocol This assay is performed to detect ubiquitylated proteins in yeast. Yeast that have been transformed with a vector expressing polyhistidine-tagged ubiquitin (Ub) under the control of a copper-inducible promoter are grown, induced with copper, and harvested. Total ubiquitylated proteins are then recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.
Yeast Protein Extracts Protocol Protocol is a simple, reliable method for the preparation of yeast protein extracts for analysis by polyacrylamide gel electrophoresis (PAGE) and Western blotting.
