Protocol describes large-scale yeast RNA isolation. It is designed to yield more than 10 mg of total nucleic acid and 300-400 µg of poly(A)-selected mRNA from each 500-ml culture. - [Read Yeast RNA Isolation: Large-Scale Protocol]
Protocol describes rapid, small-scale yeast RNA isolation. It is based on the work of Schmitt et al. (1990). Note that all containers should be washed in Rnase Away (Invitrogen) or dry baked for 24 hours at 160°C. - [Read Yeast RNA Isolation: Small-Scale Protocol]