Yeast PCR Protocols

Protocols and information related to Yeast PCR.

Protocols » Yeast Protocols » Yeast Nucleic Acid Protocols » Yeast PCR Protocols

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96-Well Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/96_well_confirmation.html

Protocol for 96-well confirmation Yeast PCR. Includes: Clonal purification; Generate a master plate (96-well format); Making a frozen backup stock; Confirmation PCR for one Row; ORF Specific Confirmation PCR --> "A-B" primers (upstream junction); Transfer template DNA to multiwell PCR plate; Prepare and dispense master mix for A-B PCR. - [Read 96-Well Confirmation Yeast PCR Protocol]

Analyzing Yeast Colonies by PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4015

Yeast colonies are suspended in complete PCR buffer and transferred to a thermal cycler for 35 cycles of PCR. The products of the amplification reaction are analyzed by gel electrophoresis. - [Read Analyzing Yeast Colonies by PCR Protocol]

Direct PCR from Whole Yeast Cells Protocol - http://www.duke.edu/web/ceramide/protocols/0003.html

Protocol for direct PCR from whole Yeast cells. - [Read Direct PCR from Whole Yeast Cells Protocol]

Gene Disruption via PCR Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/pcrko.html

Protocol for gene disruption via PCR. - [Read Gene Disruption via PCR Protocol]

Identification of End Clones in YAC Subclone Libraries Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast10.html

To identify the YAC subclones containing both a human insert and a portion of either the left or right arm of the pYAC4 vector. Identification of these clones is necessary in order to do YAC chromosome walking, and is also useful in the determination of whether a particular YAC clone has a contiguous human insert or whether a co-cloning event has occurred. Vector arm sequences are identified using pBR322 fragments from a BamHI-PvuII double digest. - [Read Identification of End Clones in YAC Subclone Libraries Protocol]

Oligonucleotide Details for PCR - http://sequence-www.stanford.edu/group/yeast_deletion_project/Primer_specs.html

Information for oligonucleotide details required for PCR. Includes: Primer choice; UPTAG PRIMER; DNTAG PRIMER; UP_45 and DOWN_45 PRIMERS; UP_90 and DOWN_90 PRIMERS. - [Read Oligonucleotide Details for PCR]

Single Tube Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Protocol for single tube confirmation yeast PCR. Includes: Clonal purification; Zymolyase treatment; PCR reactions; PCR conditions; Agarose gel electrophoresis. - [Read Single Tube Confirmation Yeast PCR Protocol]

Vectorette PCR of Yeast DNA Protocol - http://genomics.princeton.edu/botstein/protocols/vectorette.html

Protocol for vectorette PCR of Yeast DNA. - [Read Vectorette PCR of Yeast DNA Protocol]

Yeast Colony PCR - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4170

This method for yeast colony PCR is used to test for the presence of target sequences in cells. - [Read Yeast Colony PCR]

Yeast Colony PCR Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/ypcr.html

Protocol for Yeast colony PCR. Includes: Quick SDS extraction protocol. - [Read Yeast Colony PCR Protocol]