Yeast DNA Isolation Protocols

DNA Prep for CGH Protocol Protocol for DNA prep for CGH.

DNA Preparation from 10-mL Yeast Culture Protocol Protocol for DNA preparation from 10-mL Yeast culture.

High Molecular Weight Yeast Liquid DNA Preparation Protocol Protocol that isolates intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. Using this protocol one can expect a yield of 100-200 µg of DNA per prep.

Isolation of Plasmid DNA from Yeast Cells: A Ten-Minute Preparation Protocol describes the purification release of plasmid DNA from yeast cells. The resulting plasmid DNA can be used to transform Escherichia coli or yeast.

Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol Protocol (modified from Hoffman and Winston 1987) describes the purification of genomic DNA from yeast cells.

Preparation of Genomic DNA from Yeast Using Glass Beads Protocol Protocol describes the preparation of genomic DNA from a 5-ml liquid culture of yeast cells.

Preparation of Yeast DNA Embedded in Agarose Plugs Protocol Protocol for preparation of Yeast DNA embedded in agarose plugs.

Rapid Isolation of Yeast DNA Protocol This method is used to isolate genomic yeast DNA or shuttle plasmids that replicate in both S. cerevisiae and E. coli. The DNA can be used as a template for PCR and for transformation.

Small-scale Preparations of Yeast DNA Protocol This method reproducibly yields several micrograms of yeast DNA that can be efficiently cleaved by restriction enzymes and used as a template in PCR.

Yeast Chromosomal DNA Prep Protocol Protocol for Yeast chromosomal DNA prep.

Yeast DNA Isolation: Midiprep Protocol Protocol for Yeast DNA isolation. Protocol describes the preparation of DNA from a 40-ml liquid culture.

Yeast DNA Isolation: Miniprep Protocol Protocol describes the preparation of DNA from a 5-ml liquid culture of yeast.

Yeast Miniprep (Plasmid Rescue) Protocol Protocol for Yeast miniprep (plasmid rescue).