YACs Protocols

Protocols and information related to YACs.

Protocols » Yeast Protocols » Yeast Genetics Protocols » YACs Protocols

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Growth of S. cerevisiae and Preparation of DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3298

Protocol describes methods for isolation of DNA from a strain of S. cerevisiae carrying a recombinant YAC. Because the linear YAC DNAs are sensitive to shearing forces, pipettes with wide-bore tips should be used to transfer DNAs. The method is suitable for preparing DNA that will be used for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that do not require intact high-molecular-weight DNA. - [Read Growth of S. cerevisiae and Preparation of DNA Protocol]

Large-Scale Preparation of Agarose Plugs of Yeast DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4392

Protocol describes the use of agarose plugs for isolation of yeast artificial chromosome (YAC) DNA. The DNA can then be run on a pulsed-field gel and used for microinjection to produce transgenic mice. - [Read Large-Scale Preparation of Agarose Plugs of Yeast DNA Protocol]

Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4393

Protocol describes a method for purification of YAC DNA using ultrafiltration. This is the fastest method available, but care needs to be taken not to damage the DNA. The purified DNA can then be used for microinjection. - [Read Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol]

Restriction Digests of High Molecular Weight Yeast DNA Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast5.html

Protocol for restriction digests of high molecular weight yeast DNA. - [Read Restriction Digests of High Molecular Weight Yeast DNA Protocol]

Restriction Digests of YACs in Agarose Plugs Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast6.html

Protocol for restriction digests of YACs in agarose plugs. - [Read Restriction Digests of YACs in Agarose Plugs Protocol]

Screening YAC Libraries Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast7.html

Protocol for screening YAC libraries. - [Read Screening YAC Libraries Protocol]

Sizing of YAC Clones Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast8.html

Protocol for sizing of YAC clones. To determine the size of a yeast artificial chromosome within the background of the normal yeast chromosomal complement. - [Read Sizing of YAC Clones Protocol]

Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast9.html

Protocol for the subcloning of Yeast artificial chromosome into phage lambda. To subclone the large insert fragment of human DNA contained within a YAC into a bacteriophage lambda vector. The subclones are 15 to 23 kb in size, and can be used to identify new polymorphic markers from a known region of the genome, to map a specific locus, and/or to screen other libraries. - [Read Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol]

Working with Yeast Artificial Chromosomes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3297

Protocol outlines a procedure to validate and store yeast strains carrying YACs. - [Read Working with Yeast Artificial Chromosomes Protocol]

YAC Transfer by KAR1Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/YACTransfer.html

Protocol for YAC transfer by KAR1. Includes: YAC transfer by Kar1 mating; Transfer YAC from donor cells into YPH 925; Transfer YAC from YPH925 into recipient cells. - [Read YAC Transfer by KAR1Protocol]