Yeast Genetics Protocols

Protocols and information related to yeast genetics. Including: B-Galactosidase Assays; YACs; Yeast Replication Timing.

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Yeast Genetics Protocols Categories

B-Galactosidase Assay Protocols (11)

YACs Protocols (10)

Yeast Replication Protocols (3)

Yeast Genetics Protocols Protocol Links Sort by: Hits | Alphabetical

6-Azauracil Sensitivity Assay for Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4613

Protocol describes an assay where it requires growing saturated cultures of yeast, counting, and spotting serial dilutions of yeast on both CSM and CSM + 6AU plates. - [Read 6-Azauracil Sensitivity Assay for Yeast Protocol]

Curing Strains of Endogenous 2-Micron Plasmid Protocol - http://ygac.med.yale.edu/mtn/reagent/avail_reagents/curing2micron_info.stm

Protocol for curing strains of endogenous 2-micron plasmid. - [Read Curing Strains of Endogenous 2-Micron Plasmid Protocol]

EMS Mutagenesis of Yeast Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/ems_mutagenesis.html

Protocol for EMS mutagenesis of yeast. - [Read EMS Mutagenesis of Yeast Protocol]

Ethyl Methane Sulfonate (EMS) Mutagenesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4180

Protocol describes mutagenesis of yeast with ethyl methane sulfonate (EMS). It causes approximately 40-70% cell death in most haploid laboratory strains, a level of cell killing that is commonly used in mutant hunts with haploid strains. - [Read Ethyl Methane Sulfonate (EMS) Mutagenesis Protocol]

Genome-Wide Expression Protocol - http://web.wi.mit.edu/young/expression/tech.html

Protocol for genome-wide expression. - [Read Genome-Wide Expression Protocol]

Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae - http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/index.html

Introduction of the of genetics and molecular biology of the Yeast Saccharomyces cerevisiae. - [Read Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae]

Performing a Hunt by Interaction Mating Protocol - http://proteome.wayne.edu/Matinghunt.html

When more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt by interaction mating. In this protocol one strain is transformed with library DNA and the transformants are collected and frozen in aliquots. - [Read Performing a Hunt by Interaction Mating Protocol]

Plate Assay for Carboxypeptidase Y in Yeast Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4161

Protocol for plate assay for carboxypeptidase Y in Yeast cells. - [Read Plate Assay for Carboxypeptidase Y in Yeast Cells Protocol]

Quantitative Mating Assay Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/qma.html

Protocol for quantitative mating assay. - [Read Quantitative Mating Assay Protocol]

Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/missegscreen.html

Protocol for a rapid screen for chromosome missegregation mutants by DAPI staining. - [Read Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol]

UV Mutagenesis Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeastUV.html

Protocol for UV mutagenesis. - [Read UV Mutagenesis Protocol]