Yeast Protocols

Protocols and information related to yeast. Including: Yeast genetics; Yeast transformation; Yeast Protein; Yeast Nucleic Acid.

Protocols » Yeast Protocols

Yeast Protocols Categories

Yeast Cell Synchrony Protocols (6)

Yeast Nucleic Acid Protocols (7)

Yeast Transformation Protocols (10)

Yeast Two-Hybrid Systems Protocols (5)

Yeast Protocols Protocol Links Sort by: Hits | Alphabetical

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Protocol for actin capture assay. - [Read Actin Capture Assay Protocol]

Frozen Yeast Transformation Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/Frozen.html

This Protocol allows You to prepare Frozen Yeast cells that are competent for transformation after thawing. - [Read Frozen Yeast Transformation Protocol]

Handling and Using Insertion Libraries - http://ygac.med.yale.edu/mtn/protocol.stm

Handling and using insertion libraries. Includes: Curing strains of endogenous 2-micron; mTn-lacZ/LEU2 insertion library; mTn-3xHA/lacZ insertion library; mTn-3xHA/GFP insertion library. - [Read Handling and Using Insertion Libraries]

In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1831

Protocol for in vitro endoplasmic reticulum to golgi transport reaction in Yeast. Includes: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes. - [Read In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast Protocol]

Logarithmic Yeast Growth Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4179

Protocol for logarithmic Yeast growth. - [Read Logarithmic Yeast Growth Protocol]

Measuring Yeast Cell Density by Spectrophotometry Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4186

Simple method for determining the cell density of yeast cultures. - [Read Measuring Yeast Cell Density by Spectrophotometry Protocol]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Protocol for microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in Yeast. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport]

Pheromone Halo Assay Protocol - http://www.med.unc.edu/~hdohlman/haloassay.html

Protocol for pheromone halo assay. - [Read Pheromone Halo Assay Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Protocol for Yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Plasma membranes are isolated from the yeast Saccharomyces cerevisiae. The cell wall is initially digested by helicase, followed by hypoosmotic lysis and homogenization. Membranes are prepared by subsequent differential centrifugation. The activity of the H+-ATPase is then determined by measuring the amount of inorganic phosphate released from ATP. - [Read Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol]