Yeast Protocols

Actin Capture Assay Protocol Protocol for actin capture assay.

Frozen Yeast Transformation Protocol This Protocol allows You to prepare Frozen Yeast cells that are competent for transformation after thawing.

Handling and Using Insertion Libraries Handling and using insertion libraries. Includes: Curing strains of endogenous 2-micron; mTn-lacZ/LEU2 insertion library; mTn-3xHA/lacZ insertion library; mTn-3xHA/GFP insertion library.

In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast Protocol Protocol for in vitro endoplasmic reticulum to golgi transport reaction in Yeast. Includes: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes.

Logarithmic Yeast Growth Protocol Protocol for logarithmic Yeast growth.

Measuring Yeast Cell Density by Spectrophotometry Protocol Simple method for determining the cell density of yeast cultures.

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Protocol for microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in Yeast.

Pheromone Halo Assay Protocol Protocol for pheromone halo assay.

Yeast Cell Cycle by Flow Cytometry Protocol Protocol for Yeast cell cycle by flow cytometry.

Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol Plasma membranes are isolated from the yeast Saccharomyces cerevisiae. The cell wall is initially digested by helicase, followed by hypoosmotic lysis and homogenization. Membranes are prepared by subsequent differential centrifugation. The activity of the H+-ATPase is then determined by measuring the amount of inorganic phosphate released from ATP.