Yeast Sporulation Protocols

Protocols and information related to yeast sporulation.

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Alternate Sporulation Method for BY4743 Protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/spo_riles

Protocol for an alternative method for sporulation for BY4743. - [Read Alternate Sporulation Method for BY4743 Protocol]

Determining Plating Efficiency in Yeast: Direct Method - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4185

Direct method for determining efficiency in yeast. The plating efficiency of a strain is a measure of the percentage of cells in a culture that are capable of forming colonies (colony forming . - [Read Determining Plating Efficiency in Yeast: Direct Method]

Determining Plating Efficiency in Yeast: Indirect Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4184

Protocol for the indirect method of determining plating efficiency in yeast. - [Read Determining Plating Efficiency in Yeast: Indirect Method Protocol]

HC Dropout Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/yeast/ysup.html

HC Dropout Plate Supplementation Table. - [Read HC Dropout Plate Supplementation Table]

Making a Tetrad Dissection Needle Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4182

Microneedles attached to micromanipulators are used in the dissection of tetrads, isolation of zygotes from populations of mating haploid cells, and manipulation of individual cells. - [Read Making a Tetrad Dissection Needle Protocol]

Picking Zygotes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4183

Zygotes can be identified by their unique morphology. They can be easily separated away from nonmated cells using a micromanipulator. This method provides an alternative to the selection of diploid cells on a medium that prevents the growth of haploid parent cells. - [Read Picking Zygotes Protocol]

Random Spore Analysis in Yeast Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4162

Random spore analysis is used to examine meiotic recombination without performing tetrad analysis. - [Read Random Spore Analysis in Yeast Protocol]

Spore Germination Protocol - http://www-rcf.usc.edu/~forsburg/spores.html

Protocol for spore germination. This procedure is typically used for the isolation and preparation of spores from a diploid strain heterozygous for a marked disruption (e.g., yfg1::his3+) Inoculation of the spore population into minimal medium lacking the nutritional supplement corresponding to the disruption marker (e.g., minimal medium lacking histidine) allows only the disruption spores to germinate. - [Read Spore Germination Protocol]

Sporulation and Dissection of Yeast a/alpha Diploids Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_sporulation.html

Protocol for sporulation and dissection of yeast a/alpha diploids. - [Read Sporulation and Dissection of Yeast a/alpha Diploids Protocol]

Sporulation and Tetrad Dissection Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeastsporulation.html

Protocol for sporulation and tetrad dissection. - [Read Sporulation and Tetrad Dissection Protocol]

Sporulation Guide - http://www.genomics.princeton.edu/dunham/sporulationdissection.htm

Protocols and Information on sporulation. Includes: SPO++ media; 10X amino acid stock; Sporulation; Dissection. - [Read Sporulation Guide]

Sporulation Protocols - http://www.fhcrc.org/science/labs/gottschling/yeast/spo.html

Protocols for sporulation. Includes yeast vivisection. - [Read Sporulation Protocols]

Tetrad Dissection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4181

This protocol describes dissection of yeast tetrads. Tetrad analysis is useful for linkage studies and for constructing strains for genetic and biochemical experiments. - [Read Tetrad Dissection Protocol]