Xenopus Injection Protocols

Protocols and information related to Xenopus injection.

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Differences in Injection of X. laevis and X. tropicalis - http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/differences.html

Differences in injection of X. laevis and X. tropicalis. Includes: X. tropicalis lays eggs about 4 hours after a boost of hCG; In vitro fertilization is not nearly as efficient in trops compared to laevis; X. tropicalis embryos are much softer than X. laevis embryos; X. tropicalis embryos whose jelly coats are removed by cysteine have a loose sticky vitelline membrane; X. tropicalis do not have a "summer slump". - [Read Differences in Injection of X. laevis and X. tropicalis]

Making and Injecting Xenopus Embryos Protocol - http://spot.colorado.edu/~klym/Methods/embryos.htm

Protocol for making and injecting Xenopus embryos. Includes: Injections; Egg Activation and Inversion Experiments; - [Read Making and Injecting Xenopus Embryos Protocol]

Natural and Artificial Fertilization of Xenopus Eggs Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/Pdfs/Natural%20and%20Artificial%20Fertilization%20of%20Frog%20Eggs.pdf

Protocol for natural and artificial fertilization of Xenopus eggs. - [Read Natural and Artificial Fertilization of Xenopus Eggs Protocol]

Xenopus Microinjection Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html

Protocol for Xenopus microinjection. - [Read Xenopus Microinjection Protocol]