Xenopus Egg Extracts Protocols

Protocols and information related to Xenopus egg extracts.

Protocols » Xenopus Protocols » Xenopus Egg Extracts Protocols

Xenopus Egg Extracts Protocols Protocol Links Sort by: Hits | Alphabetical

Crude Depletion Conditions for XKCM1 Protocol - http://mitchison.med.harvard.edu/protocols/ext1.html

Protocol for crude depletion conditions for XKCM1. - [Read Crude Depletion Conditions for XKCM1 Protocol]

CSF Extract Prep for Xenopus Spindle Assembly Protocol - http://mitchison.med.harvard.edu/protocols/ext3.html

Protocol for CSF extract prep for Xenopus spindle assembly. - [Read CSF Extract Prep for Xenopus Spindle Assembly Protocol]

Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1422

Protocol for cytostatic factor extract preparation for spindle assembly. The quality of the eggs is essential for good CSF extracts. Always sacrifice quantity for quality when trying to make functional extracts. Discard any batches of eggs that have "puff balls" or activated eggs that constitute more than 10% of the eggs. Use laid eggs and collect eggs at about 16 to 17 hours after priming with Progesterone (found in Pregnant Mare Serum Gonadotropin (PMSG)). - [Read Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol]

HSS Depletion Conditions for XKCM1 Protocol - http://mitchison.med.harvard.edu/protocols/ext2.html

Protocol for HSS depletion conditions for XKCM1. - [Read HSS Depletion Conditions for XKCM1 Protocol]

Preparation of Xenopus Sperm Nuclei Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p877

Protocol describes the preparation of nuclei from Xenopus sperm. These nuclei can be used for spindle assembly assays, microscopy, or DNA synthesis. - [Read Preparation of Xenopus Sperm Nuclei Protocol]

Xenopus Egg CSF Extract Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1049

Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation. - [Read Xenopus Egg CSF Extract Preparation Protocol]

Xenopus Sperm Nuclei Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1050

Protocol for Xenopus sperm nuclei preparation. Sperm Nuclei can be made and stored as a stock for later use with Cytostatic Factor Extracts. - [Read Xenopus Sperm Nuclei Preparation Protocol]