Viral Isolation Protocols

Protocols and information related to viral isolation. Including isolation of Adenovirus and Retrovirus.

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Viral Isolation Protocols Protocol Links Sort by: Hits | Alphabetical

A Protocol for AAV Vector Production and Purification - http://www.brc.riken.jp/lab/dna/rvd/SOP/AAV/AAVProtocol.pdf

Protocol describes typical methods that are used to propagate and purify AAV vectors for experiments both in vitro and in vivo. Includes: Principles of the Triple Plasmid Transfection System; Plasmids; Transfection and Extraction of Virus; Purification of the AAV vector. - [Read A Protocol for AAV Vector Production and Purification]

A Simplified System for Rapid Generation of Recombinant Adenoviruses - http://www.coloncancer.org/adeasy/protocol.htm

A simplified system for rapid generation of recombinant adenoviruses. Includes: Generation of Recombinants in Bacterial Cells; Viral Production in 293 or 911 Cells; Preparation of High Titer Viral Stocks. - [Read A Simplified System for Rapid Generation of Recombinant Adenoviruses]

Adenovirus DNA Replication Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/AdDNAreplication.html

Protocol describes large fragment DNA replication by Adenovirus proteins using TP-DNA as template. - [Read Adenovirus DNA Replication Assay Protocol]

Adenovirus Protocol - http://www.mc.vanderbilt.edu/root/vumc.php?site=mgcore&doc=4301

Protocol for Adenovirus. - [Read Adenovirus Protocol]

Infection Protocol for Adherent Cell Types - http://www.med.umich.edu/vcore/protocols/RetroviralCellScreenInfection101705.pdf

This protocol may be used for the infection of adherent cell lines. - [Read Infection Protocol for Adherent Cell Types]

Isolation of Genomic and Viral DNA from Lymphocytes Protocol - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Protocol for the isolation of genomic and viral DNA from lymphocytes. Includes: Viral DNA in cytosolic fraction and Viral DNA in nuclear fraction integrated. - [Read Isolation of Genomic and Viral DNA from Lymphocytes Protocol]

Isolation of Infectious HIV-1 Protocol - http://www.miltenyibiotec.com/skripte/pdf.php?mm_id=516&session_id=2fcdee70b1da5252291e83a27e1c76a9&ip=222.165.176.129

Protocol for the isolation of infectious HIV-1. Includes: HIV-1 capture strategy; Protocol for the isolation of HIV-1 virions; Magnetic labeling; Magnetic separation; Elution option A for virion lysate; Elution option B for intact virions. - [Read Isolation of Infectious HIV-1 Protocol]

Kit for Rapid Enrichment of SARS Virus - http://www.gentaur.com/sars_isolation.htm

During incubation period and the course of disease, SARS virus replicates and releases from cells. The level of virus varies greatly from sample to sample. At early stage of incubation period or during the late convalescent phase, the concentration of virus is very low in all kinds of samples. Virus is also detected at extremely low concentrations in plasma during... - [Read Kit for Rapid Enrichment of SARS Virus]

Plaque Assays for Adenovirus Titration Protocol - http://www.whitelabs.org/Lab%20Protocols/adenovirus%20protocols/PLAQUE%20ASSAYS%20FOR%20ADENOVIRUS%20TITRATION.htm

Protocol for plaque assays for adenovirus titration. - [Read Plaque Assays for Adenovirus Titration Protocol]

Preparation of High Titer Adenovirus in P11 Cells Protocol - http://www.whitelabs.org/Lab%20Protocols/adenovirus%20protocols/Preparation%20of%20High%20Titer%20Adenovirus%20in%20P11%20cells.htm

Protocol for the preparation of high titer adenovirus in P11 cells. - [Read Preparation of High Titer Adenovirus in P11 Cells Protocol]

Retroviral Infection of Primary Human Keratinocytes (PHKs) Protocol - http://www.methodbook.net/cellcult/infectn.html

Protocol for the retroviral infection of primary human keratinocytes (PHKs). - [Read Retroviral Infection of Primary Human Keratinocytes (PHKs) Protocol]

Use of “293-GPG” cells to Generate Amphotropic Retroviruses, plus Infection Protocol - http://www.flemingtonlab.com/Protocols/293amphMaintProdInfection.pdf

Use of “293-GPG” cells to generate amphotropic retroviruses, plus infection protocol. - [Read Use of “293-GPG” cells to Generate Amphotropic Retroviruses, plus Infection Protocol]