Stem Cell Injection Protocols

Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips.

Collecting Two-Cell- to Compacted Morula-Stage Embryos Protocol This protocol describes a method for collecting two-cell- to compacted morula-stage embryos. Such embryos can then be used for microinjection.

Injecting Blastocysts Protocol This protocol describes a method for injecting mouse blastocysts with embryonic stem (ES) cells to produce chimeras.

Preparation of Embryonic Stem (ES) Cells for Injection Protocol Injection of ES cells into the cavity of blastocysts is one of the main methods for generating chimeras. This protocol describes the preparation of ES cells for injection.

Subcutaneous Injection of Embryonic Stem (ES) Cells Protocol This protocol describes a method for subcutaneous injection of ES cells into mice.