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Protocols and information related to stem cell injection.
Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips. - [Read Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol]
This protocol describes a method for collecting two-cell- to compacted morula-stage embryos. Such embryos can then be used for microinjection. - [Read Collecting Two-Cell- to Compacted Morula-Stage Embryos Protocol]
This protocol describes a method for injecting mouse blastocysts with embryonic stem (ES) cells to produce chimeras. - [Read Injecting Blastocysts Protocol]
Injection of ES cells into the cavity of blastocysts is one of the main methods for generating chimeras. This protocol describes the preparation of ES cells for injection. - [Read Preparation of Embryonic Stem (ES) Cells for Injection Protocol]
This protocol describes a method for subcutaneous injection of ES cells into mice. - [Read Subcutaneous Injection of Embryonic Stem (ES) Cells Protocol]
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