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Science, at bottom, is really anti-intellectual. It always distrusts pure reason, and demands the production of objective fact. ~H.L. Mencken, Minority Report: H.L. Mencken's Notebook, 1956
Protocols and information related to stem cell injection.
| Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4417 Injection needles require sharp tips that allow the blastocyst to be penetrated easily. This protocol describes a method of creating sharp injection needle tips. - [Read Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles Protocol] |
| Collecting Two-Cell- to Compacted Morula-Stage Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4359 This protocol describes a method for collecting two-cell- to compacted morula-stage embryos. Such embryos can then be used for microinjection. - [Read Collecting Two-Cell- to Compacted Morula-Stage Embryos Protocol] |
| Injecting Blastocysts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4418 This protocol describes a method for injecting mouse blastocysts with embryonic stem (ES) cells to produce chimeras. - [Read Injecting Blastocysts Protocol] |
| Preparation of Embryonic Stem (ES) Cells for Injection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4416 Injection of ES cells into the cavity of blastocysts is one of the main methods for generating chimeras. This protocol describes the preparation of ES cells for injection. - [Read Preparation of Embryonic Stem (ES) Cells for Injection Protocol] |
| Subcutaneous Injection of Embryonic Stem (ES) Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4383 This protocol describes a method for subcutaneous injection of ES cells into mice. - [Read Subcutaneous Injection of Embryonic Stem (ES) Cells Protocol] |