Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Protocols and information related to mouse embryonic fibroblasts.
| ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp5.html Protocol for ES/MEF cell culture and electroporation of targeting construct. - [Read ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol] |
| Propagation of Pluripotent Mouse Embryonic Stem (ES) Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4484 This protocol describes the culture of embryonic stem (ES) cells using mitotically inactivating primary mouse embryonic fibroblast (MEF) cells as a feeder layer (preparation described in Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates). The ES culture medium is supplemented with recombinant leukemia inhibitory factor (LIF) to help maintain the cells as pluripotent stem cells. This protocol has been optimized for the ES-D3 cell line. - [Read Propagation of Pluripotent Mouse Embryonic Stem (ES) Cells Protocol] |
| Splitting Human ES cells on MEFs Protocol - http://ink.primate.wisc.edu/~thomson/pdfs/splitting_es.pdf Protocol for splitting human ES cells on MEFs. - [Read Splitting Human ES cells on MEFs Protocol] |
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