Proteomics, peptide identification, 2D 2-D gel electrophoresis, proteome, protein identification, mass spectrometry, mass spec, methods in proteomics, techniques for proteomic protein identification.
In-Gel Digestion Protocol. Excision of protein bands (spots) from polyacrylamide gels Reduction and alkylation . Reduction and alkylation . MALDI analysis of the supernatant after in-gel digestion. Extraction of Peptides.Matthias Wilm EMBL Bioanalytical R - [Read In-gel digestion of proteins to be analyzed by mass spectrometry]