Proteomics, peptide identification, 2D 2-D gel electrophoresis, proteome, protein identification, mass spectrometry, mass spec, methods in proteomics, techniques for proteomic protein identification.
Coomasie and SYPRO Destaining Method Coomasie and SYPRO Destaining Method Protocol. MSCLS Center for Mass Spectrometry and Proteomics
In Gel Digestion Protocols for Silver Stained and Coomassie Stained Gels In Gel Digestion Protocols for Silver Stained and Coomassie Stained Gels. (EMBL protocol).
In-gel digestion of proteins to be analyzed by mass spectrometry In-Gel Digestion Protocol. Excision of protein bands (spots) from polyacrylamide gels Reduction and alkylation . Reduction and alkylation . MALDI analysis of the supernatant after in-gel digestion. Extraction of Peptides.Matthias Wilm EMBL Bioanalytical R
In-gel Digestion protocol In-gel Digestion protocol. BA Stanley.
Millipore ZipTip detailed protocol. MSCLS ZipTip Protocol detailed protocol. MSCLS Center for Mass Spectrometry and Proteomics
Other MALDI-TOF Sample Prep Protocols Other MALDI-TOF Sample Prep Protocols. BA Stanley.
Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis. Univ. Virginia.
Protein Identification Strategy Diagram Protein Identification Strategy Diagram. A great picture of stregies for protein id. PMSC Toronto
Proteomic Protocols for Sample Submission to Mass Spec Proteomic Protocols for Sample Submission to Mass Spec. Gel Staining, In gel trypsin digestion, Coomassie Blue staining and TCA precipitation. CFG U. Albany.
Simple In-gel Digestion Protocol In-gel Digestion Protocol. BA Stanley.
UCSF In-Gel Digest Procedure UCSF In-Gel Digest Procedure. Al Burlingame
DNA Protocols - DNA Cloning
Cell Biology Protocols - Cell Culture Protocols
PCR Protocols Protein Protocols
Western Blot Protocols
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