Tissue Preparation for LCM Protocols

Protocols and information related to tissue preparation for Laser Capture Microdissection (LCM).

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Tissue Preparation for LCM Protocols Protocol Links

(LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cel LCM isolates specific cells or tissues from samples mounted on microscope slides. The samples are viewed through a thermoplastic film that is attached to a microcentrifuge tube lid. Localized heat, caused by the application of a laser pulse, fuses the membrane to the cells of interest, which can then be harvested for further analysis. RNA and proteins can be purified from the isolated cells, allowing detailed analysis of gene expression. This protocol is divided into three stages.

70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol Protocol for 70% ethanol fixation for recovery of DNA, RNA, and protein.

Low-melt Polyester Embedding Protocol Protocol for low-melt polyester embedding. Includes: Large Tissue Specimens; Small Tissue Specimens and Biopsies; Histology Slide Cutting Procedure.

Paraffin Embedding Protocol Protocol for paraffin embedding.

Slide Preparation for Laser Capture Microdissection (LCM) Protocol Protocol for slide preparation for laser capture microdissection (LCM) for subsequent DNA, RNA, and protein analysis.