Tissue Preparation for LCM Protocols Protocol Links
(LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cel LCM isolates specific cells or tissues from samples mounted on microscope slides. The samples are viewed through a thermoplastic film that is attached to a microcentrifuge tube lid. Localized heat, caused by the application of a laser pulse, fuses the membrane to the cells of interest, which can then be harvested for further analysis. RNA and proteins can be purified from the isolated cells, allowing detailed analysis of gene expression. This protocol is divided into three stages.