Primary Cell Isolation and Culture Protocols

Protocols and information related to primary cell isolation and culture. Including: Mammalian Cell Culture; Laser Capture Microdissection.

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Primary Cell Isolation and Culture Protocols Categories

Laser Capture Microdissection Protocols (15)

Mammalian Cell Culture Protocols (27)

Primary Cell Isolation and Culture Protocols Protocol Links Sort by: Hits | Alphabetical

70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/EthanolFixation.html

Protocol for 70% ethanol fixation for recovery of DNA, RNA and protein. - [Read 70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol]

Low-melt Polyester Embedding Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/LowMeltPolyester.html

Protocol for low-melt polyester embedding. - [Read Low-melt Polyester Embedding Protocol]

Manual Microdissection - http://cgap-mf.nih.gov/Protocols/Microdissection/ManualMicrodissection.html

There are several manual methods that can be used to perform tissue microdissection. Techniques using hand-held tools as well as mechanical micromanipulator-based approaches have been described. However, speed and precision are the most important parameters and any method that achieves these is adequate. Investigators should also expect to invest time initially by practicing on 10 to 20 cases to begin to feel comfortable with the technique. - [Read Manual Microdissection]

Microdissection Overview - http://cgap-mf.nih.gov/Protocols/Microdissection/Overview.html

Overview of microdissection. Includes: The Goals of Microdissection; The Development of Microdissection. - [Read Microdissection Overview]

Paraffin Embedding Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/ParaffinEmbedding.html

Protocol for paraffin embedding. - [Read Paraffin Embedding Protocol]

Protein Staining Protocols - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/StainingProcedures.html

Protocols for protein staining. Includes: Coomassie Blue Staining; Silver Nitrate Staining; Zinc Imidazole Negative Staining. - [Read Protein Staining Protocols]

Slide Preparation for Manual Microdissection Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/SlidePrepManualMicrodissection.html

Slide Preparation for Manual Microdissection for Subsequent DNA, RNA, and Protein Analysis. Manual microdissection and subsequent molecular analysis can be carried out on slides stained using standard hematoxylin and eosin methods. However, if cell types that are (or are not) expressing a specific protein are required for a study, then more advanced slide preparation methods such as Immuno-LCM may be utilized. - [Read Slide Preparation for Manual Microdissection Protocol]

Static Culture of Postimplantation Embryos for Imaging Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4459

This protocol describes a method for static culture of early postimplantation mouse embryos on a microscope stage. Embryos between 6.5 and 9.5 days post coitum (dpc) can be cultured and imaged for 24 hours, with very little growth retardation. - [Read Static Culture of Postimplantation Embryos for Imaging Protocol]

Targeted Differential Display Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/RNA/TargetedDifferentialDisplay.html

The protocol is one example of differential gene expression analysis of cells obtained from microdissected tissue. Includes: Microdissection and RNA Isolation; Reverse Transcription; PCR; P.A.G.E.; Sequencing of Differentially Expressed Bands. - [Read Targeted Differential Display Protocol]

The Isolation and Culture of Rat Hepatic Cells Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/7.htm

This method enables the isolation of different populations of liver cells and describes their subsequent culture. - [Read The Isolation and Culture of Rat Hepatic Cells Protocol]

Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells - http://www.natureprotocols.com/2006/11/17/thymosin_4_t4induced_outgrowth.php

Thymosin β4 (Tβ4)-induced outgrowth and differentiation of vasculogenic precursor cells. - [Read Thymosin β4 (Tβ4)-induced Outgrowth and Differentiation of Vasculogenic Precursor Cells]