Gel Elongation Assay for Type II Fatty Acid Synthesis Protocol To determine the selectivity of the inhibitors identified during screening efforts we developed gel-elongation assay using crude bacterial lysate directly to determine the target specificities of fatty acid synthesis inhibitors.
High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents. Method is highly sensitive, provides quantifiable data and offers high-throughput screening capability. Starting with the treatment of cells with different doses of damaging agents, pre-prepared growing media containing 96-well plates are inoculated and cell population is automatically monitored every 10 min for 48 hours.
Human Thyroid Culture Protocol Protocol enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland.
Image Based Assay of Endothelial Cell Tube Formation Protocol Protocol describes an assay that relies on the endothelial cells’ ability to form distinct blood-vessel-like tubules in an extracellular matrix where they can subsequently be visualized by fluorescence microscopy. Although quantification of the tubules can be performed by manual tracing, this method precludes the use of the assay in unbiased high-throughput applications.
SOLVO In Vitro Transporter Assay Technology In Vitro ABC Transporter Assays for Hepato-Biliary DMPK; ABC transporter assays; Uptake transporter assays; Transporter assays for enteral absorption PK; Transporter Assays for Blood-Brain-Barrier PK; In Vitro Transporter Assays for renal excretion.
Target Specific Whole Cell Assay for Antibacterial Drug Discovery Protocol Protocol describes a target selective S. aureus whole cell assay that combines agar-diffusion and protein over expression techniques. This agar based two-plate differential sensitivity assay was used to help confirm the newly discovered antibiotic platensimycin inhibited bacterial growth by specifically targeting the essential FASII enzyme FabF6.
