Drug Discovery

Protocol for ATPase assay kit. - [Read ATPase Assay Kit Protocol]

To determine the selectivity of the inhibitors identified during screening efforts we developed gel-elongation assay using crude bacterial lysate directly to determine the target specificities of fatty acid synthesis inhibitors. - [Read Gel Elongation Assay for Type II Fatty Acid Synthesis Protocol]

High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents. Method is highly sensitive, provides quantifiable data and offers high-throughput screening capability.  Starting with the treatment of cells with different doses of damaging agents, pre-prepared growing media containing 96-well plates are inoculated and cell population is automatically monitored every 10 min for 48 hours.  - [Read High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol]

Protocol enables the culturing of thyroid cells without loss of differentiation and medium change.  It is potentially useful for the long-term study of drug effects on the thyroid gland. - [Read Human Thyroid Culture Protocol]

Protocol describes an assay that relies on the endothelial cells’ ability to form distinct blood-vessel-like tubules in an extracellular matrix  where they can subsequently be visualized by fluorescence microscopy.  Although quantification of the tubules can be performed by manual tracing, this method precludes the use of the assay in unbiased high-throughput applications. - [Read Image Based Assay of Endothelial Cell Tube Formation Protocol]

Protocol for selection of appropriate imaging equipment and methodology for live cell imaging in Drosophila. - [Read Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila]

In Vitro ABC Transporter Assays for Hepato-Biliary DMPK; ABC transporter assays; Uptake transporter assays; Transporter assays for enteral absorption PK; Transporter Assays for Blood-Brain-Barrier PK; In Vitro Transporter Assays for renal excretion. - [Read SOLVO In Vitro Transporter Assay Technology]

Protocol describes a target selective S. aureus whole cell assay that combines agar-diffusion and protein over expression techniques.  This agar based two-plate differential sensitivity assay was used to help confirm the newly discovered antibiotic platensimycin inhibited bacterial growth by specifically targeting the essential FASII enzyme FabF6. - [Read Target Specific Whole Cell Assay for Antibacterial Drug Discovery Protocol]