Genomic DNA Isolation Protocols

Protocols and information related to genomic DNA isolation.

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Genomic DNA Isolation Protocols Protocol Links Sort by: Hits | Alphabetical

DNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4098

In this protocol, the DNA-binding capacity of Wizard MagneSil particles is used to capture and release a consistent amount of DNA (100 ng) across a wide range of samples. At the end of the procedure, the DNA is eluted into 100 µl Elution Buffer to give a final concentration of 1 ng/µl, relieving the need for postpurification DNA quantitation. - [Read DNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs Protocol]

Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3695

Protocol for isolation of DNA from mammalian cells grown in 96-well microtiter plates. Each well yields sufficient genomic DNA for several standard PCRs or for analysis in a single lane of a Southern hybridization. - [Read Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Protocol for the isolation of genomic and viral DNA from lymphocytes using the QIAGEN® Genomic-tip. - [Read Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip]

Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Pilot Reactions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3978

Protocol is used to establish conditions for restriction enzyme digestion of eukaryotic genomic DNA that will generate fragments of a size appropriate for construction of genomic libraries. To construct a genomic library, the average length of the starting genomic DNA should be at least eight times the capacity of the vector. - [Read Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Pilot Reactions Protocol]

Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Preparative Reactions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3979

The results of the pilot experiment (Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Pilot Reactions) are used to establish conditions for partial digestion of eukaryotic DNA in the large-scale reactions described here. - [Read Partial Digestion of Eukaryotic DNA for Use in Genomic Libraries: Preparative Reactions Protocol]

Preparation of Genomic DNA from Bacteria Protocol - http://userpages.umbc.edu/~jwolf/m1.htm#genomic%20DNA

Protocol for preparation of genomic DNA from bacteria. - [Read Preparation of Genomic DNA from Bacteria Protocol]

Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4038

Simple protocol is used to extract DNA from small numbers of cultured cells and from fragments of soft or bony tissues. The method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs. - [Read Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol]

Rapid Method for Preparation of Genomic DNA from P. aeruginosa Protocol - http://wheat.pw.usda.gov/~lazo/methods/goldberg/genomic.html

Protocol for rapid method for preparation of genomic DNA from P. aeruginosa. - [Read Rapid Method for Preparation of Genomic DNA from P. aeruginosa Protocol]