Protocols and information related to DNA isolation and purification. Including: DNA Isolation from Mammalian Cells; DNA Plasmid Preps; Genomic DNA Isolation.
DNA Fragment Purification from Agarose or Acrylamide Protocol Protocol for DNA fragment purification from agarose or acrylamide. For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred.
DNA Isolation From BAC & PAC Clones Protocol This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary.
Fungal DNA Isolation Protocol Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml).
Lambda DNA Preparation Protocol Protocol for lambda DNA preparation. This is a plate method that gives very good yield for cloning.
Single Strand DNA Prep For Sequencing Protocol Protocol for single strand DNA prep for sequencing. This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly).
