DNA Isolation Protocols

Protocols and information related to DNA isolation and purification. Including: DNA Isolation from Mammalian Cells; DNA Plasmid Preps; Genomic DNA Isolation.

Protocols » Nucleic Acid Purification Protocols » DNA Isolation Protocols

DNA Isolation Protocols Categories

DNA Isolation from Mammalian Cells Protocols (5)

DNA Plasmid Preps Protocols (20)

Genomic DNA Isolation Protocols (8)

DNA Isolation Protocols Protocol Links Sort by: Hits | Alphabetical

Colony Hybridization Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/ColonyHybridization.html

Protocol for colony hybridization. - [Read Colony Hybridization Protocol]

DNA Extraction from Fish Fins Protocol - http://genome-lab.ucdavis.edu/Protocols/dna_extraction_from_fish_fins.htm

Protocol for DNA extraction from fish fins. - [Read DNA Extraction from Fish Fins Protocol]

DNA Extraction Technique for Biopsy Tissue Protocol - http://genome-lab.ucdavis.edu/Protocols/DNA%20extraction%20technique%20for%20biopsy%20tissue.htm

Protocol for DNA extraction technique for biopsy tissue. - [Read DNA Extraction Technique for Biopsy Tissue Protocol]

DNA Fragment Purification from Agarose or Acrylamide Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D5.html

Protocol for DNA fragment purification from agarose or acrylamide. For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose or Acrylamide Protocol]

DNA Isolation From BAC & PAC Clones Protocol - http://bacpac.chori.org/bacpacmini.htm

This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. - [Read DNA Isolation From BAC & PAC Clones Protocol]

DNA Preparation Bacterial DNA from Mucoid Strains Protocol - http://rothlab.ucdavis.edu/protocols/D03.html

Protocol for DNA preparation bacterial DNA from mucoid strains. - [Read DNA Preparation Bacterial DNA from Mucoid Strains Protocol]

Fungal DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]

Lambda DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D3.html

Protocol for lambda DNA preparation. This is a plate method that gives very good yield for cloning. - [Read Lambda DNA Preparation Protocol]

Purification of PCR Products in Preparation for Cloning Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3825

This protocol describes how to use proteinase K to destroy thermostable enzymes and to purify amplified DNA in preparation for cloning. - [Read Purification of PCR Products in Preparation for Cloning Protocol]

Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3914

Ethidium bromide is removed from DNA by phase extraction with organic solvents. - [Read Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol]

Single Strand DNA Prep For Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S2.html

Protocol for single strand DNA prep for sequencing. This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). - [Read Single Strand DNA Prep For Sequencing Protocol]