RNA Electrophoresis Protocols

Protocols and information related to electrophoresis of RNA.

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Denaturing Agarose Gel Electrophoresis of RNA Protocol - http://www.ambion.com/techlib/append/supp/rna_gel.html

Protocol for denaturing agarose gel electrophoresis of RNA. Includes: Prepare the gel; Prepare the RNA sample; Electrophoresis. - [Read Denaturing Agarose Gel Electrophoresis of RNA Protocol]

Preparation of a Denaturing Agarose Gel for the Analysis of RNA Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4127

Protocol for preparation of a denaturing agarose gel for the analysis of RNA samples. The integrity of RNA samples can be analyzed by electrophoresis on a denaturing agarose gel containing formaldehyde. - [Read Preparation of a Denaturing Agarose Gel for the Analysis of RNA Samples Protocol]

RNA Gels Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/RNAGels.pdf

Protocol for RNA gel. - [Read RNA Gels Protocol]

Separation of RNA According to Size: Electrophoresis of Glyoxylated RNA through Agarose Gels - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4057

Separation of RNAs according to size is the first stage in northern blotting and hybridization. The method described in this protocol uses glyoxal to denature the RNA, ethidium bromide to stain it, and agarose gel electrophoresis to separate the resulting glyoxal-RNA-ethidium adducts. - [Read Separation of RNA According to Size: Electrophoresis of Glyoxylated RNA through Agarose Gels]

Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formalde - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4050

Separation of RNAs according to size is the first stage in northern blotting and hybridization. The method described in this protocol uses formaldehyde to denature the RNA, ethidium bromide to stain it, and electrophoresis through agarose gels containing 2.2 M formamide to separate the resulting formaldehyde-RNA-ethidium adducts. - [Read Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formalde]

Transfer and Fixation of Denatured RNA to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4060

Protocol describes the transfer of RNA from agarose gels to neutral or positively charged nylon membranes, using upward capillary flow of neutral or alkaline buffers. RNA becomes covalently fixed to positively charged nylon membranes during transfer in alkaline buffers. However, treatment by UV irradiation or heating is required to fix RNA to neutral membranes. - [Read Transfer and Fixation of Denatured RNA to Membranes Protocol]

Visualization of RNA Preparations on 1% Agarose Gels Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000025.pdf?pid=PP00000025

Method is used to assess (roughly) the integrity of total RNA samples by visualization of discreet 18S and 28S ribosomal RNAs. Total RNA is separated by electrophoresis through a 1% agarose gel containing 1.3 ìM ethidium bromide. Binding of the ethidium bromide to the RNA allows visualization of the separated RNA molecules when the gel is exposed to ultraviolet (UV) light. - [Read Visualization of RNA Preparations on 1% Agarose Gels Protocol]