Nucleic Acid Detection Protocols

Protocols and information related to nucleic acid detection. Includes: Nucleic Acid Blotting.

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Nucleic Acid Blotting Protocols (10)

Nucleic Acid Detection Protocols Protocol Links Sort by: Hits | Alphabetical

Fluorometric Quantitation of DNA Using Hoechst 33258 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4458

Protocol describes the quantitation of DNA using Hoechst 33258, a fluorescent dye that binds to double-stranded DNA. Fluorometry is simple and more sensitive than spectrophotometry, and allows the detection of nanogram quantities of DNA. The assay can only be used to measure the concentration of DNAs whose sizes exceed ~1 kb, as Hoechst 33258 binds poorly to smaller DNA fragments. - [Read Fluorometric Quantitation of DNA Using Hoechst 33258 Protocol]

Nonradioactive Labeling and Detection Seminar - http://www.piercenet.com/presentations/nonrad/index.htm

Seminar for nonradioactive labeling and detection. - [Read Nonradioactive Labeling and Detection Seminar]

Potassium Permanganate Sensitivity Assay - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/PotassiumPermanganate.html

Protocol for potassium permanganate sensitivity assay. - [Read Potassium Permanganate Sensitivity Assay]

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Protocol for preparation of fluorescent DNA probe from HUMAN mRNA or total RNA using direct incorporation. Includes: Preparing fluoresenctly labeled cDNA (probe); Washing and Scanning Arrays. - [Read Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation]

Quick Chromatin Digestions Protocol - http://www.fhcrc.org/science/labs/tsukiyama/protocols/quick_chromatin_digestions.pdf

Protocol for qucik chromatin digestions. - [Read Quick Chromatin Digestions Protocol]

Restriction Landmark Genomic Scanning Protocol - http://www.natureprotocols.com/2007/01/25/restriction_landmark_genomic_s.php

Restriction landmark genomic scanning (RLGS) is a method to detect large numbers of restriction landmarks in a single experiment. It is based on the concept that restriction enzyme sites can serve as landmarks throughout a genome. RLGS uses direct end-labeling of the genomic DNA digested with a rare-cutting restriction enzyme and high-resolution two-dimensional electrophoresis. - [Read Restriction Landmark Genomic Scanning Protocol]