Patch Clamping Protocols

Principles behind the techniques: channel specific techniques. - [Read Channel Specific Techniques]

Combined patch clamp recording and mRNA expression profiling in individual neurons. - [Read Combined Patch Clamp Recording and mRNA Expression Profiling in Individual Neurons]

Patch clamp measurements applied to neurons. - [Read Patch Clamp Measurements Applied to Neurons]

Protocol for pulling patch electrodes. - [Read Pulling Patch Electrodes Protocol]

The cell-attached capacitance recording technique is a powerful technique that has been successfully used to resolve single vesicle fusion and fusion pore conductance.  This technique, however, has not been applied to synapses because of the difficulty in accessing release sites.  Here, we developed a technique to expose release sites in a large nerve terminal, the calyx of Held, which contains clear-core glutamatergic vesicles. - [Read The Cell Attached Capacitance Recording Technique]

Learn about the patch clamp technique. - [Read The Patch Clamp Technique]

Protocol for transcranial application of oscillating current stimulation. - [Read Transcranial Application of Oscillating Current Stimulation Protocol]

The technique of patch clamp recording was invented by Bert Sakmann and Erwin Neher in 1981 for which they received the NOBEL prize. The technique is best suited for the study of the behaviour of single ion channels, or macroscopic currents in small cells or macro-patches. The whole cell technique allows one to control the composition of solutes on both sides of the membrane. - [Read Whole Cell Patch Clamp Technique]

This article briefly summarizes the use of this variant of the patch-clamp technique in isolated cardiac myocytes with an emphasis on practical suggestions. - [Read Whole-Cell Voltage Clamp of Isolated Heart Cells]