Rat Neuronal Cell Culture Protocols

Conditional ablation of stat3/socs3 discloses the dual role for reactive astrocytes after spinal cord injury. The current protocol demonstrates that reactive astrocytes play a crucial role in wound healing and functional recovery by using mice with a selective deletion of the signal transducer and activator of transcription-3 (STAT3) or suppression of cytokine signaling-3 (SOCS3) under the control of Nestin gene promoter/enhancer (STAT3N–/–, SOCS3N–/–). - [Read Conditional Ablation of Stat3 Socs3 Discloses the Dual Role for Reactive Astrocytes]

Protocol for culturing schwann cells from adult peripheral nerve. - [Read Culturing Schwann Cells from Adult Peripheral Nerve Protocol]

Protocol for primary culture of rat cerebral astrocytes. - [Read Primary Culture of Rat Cerebral Astrocytes Protocol]

Protocol for standardization of both the isolation procedure and the quality of the cultures. - [Read Rat Chromaffin Cells Primary Cultures Standardization and Quality Assessment for Single Cell Assays]

Optimized Protocol for primary rat hippocampal or cortical neurons. - [Read Rat Neuron Nucleofector Kit]