Protocols and information related to neuronal cell culture.
Culture of Dissociated Cortical Neurons Protocol Protocol for culture of dissociated cortical neurons.
Culture of Dissociated Sympathetic Neurons Protocol Protocol for culture of dissociated sympathetic neurons.
Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation Neuron-fibroblast coculture assay to demonstrate presynaptic differentiation. Includes: Preparation of HEK293T cells, Coculture of HEK293T cells and neurons, Immunostaining, Vesicle turnover experiment, Image acquisition and quantitation.
Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture.
DNA Protocols - DNA Cloning
Cell Biology Protocols - Cell Culture Protocols
PCR Protocols Protein Protocols
Western Blot Protocols
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