Neuronal Cell Culture Protocols

Protocols and information related to neuronal cell culture.

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Neuronal Cell Culture Protocols Categories

C. elegans Neuronal Cell Culture Protocols (3)

Neuronal Cell Culture Media Protocols (1)

Rat Neuronal Cell Culture Protocols (5)

Neuronal Cell Culture Protocols Protocol Links Sort by: Hits | Alphabetical

Culture of Dissociated Cortical Neurons Protocol - http://crutcherlab.med.uc.edu/DCN.htm

Protocol for culture of dissociated cortical neurons. - [Read Culture of Dissociated Cortical Neurons Protocol]

Culture of Dissociated Sympathetic Neurons Protocol - http://crutcherlab.med.uc.edu/DSN.htm

Protocol for culture of dissociated sympathetic neurons. - [Read Culture of Dissociated Sympathetic Neurons Protocol]

Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation - http://www.natureprotocols.com/2006/09/26/neuronfibroblast_coculture_ass.php

Neuron-fibroblast coculture assay to demonstrate presynaptic differentiation. Includes: Preparation of HEK293T cells, Coculture of HEK293T cells and neurons, Immunostaining, Vesicle turnover experiment, Image acquisition and quantitation. - [Read Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol]